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Stable-light producingEscherichia coli

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Summary

Stable light production inEscherichia coli is achieved by cloning the genes encoding bacterial luciferase fromVibrio harveyi. To gain advantage of sensitive detection of light we transferred the genes under the control of a regulatable promoter system and searched for growth and buffer conditions where bacteria emitted stable light. Based on our findings an automated biosensor system can be developed to monitor the effects of biologically active compounds against stable-light producing bacteria.

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References

  • Baldwin, T.O., Berends, T., Bunch, T.A., Holzman, T.F., Rausch, S.K., Shamansky, L., Treat, M.L. and Ziegler, M.M. (1984)Biochemistry,23, 3663–3667

    PubMed  Google Scholar 

  • Dente, L., Cesareni, G. and Cortese, R. (1983)Nucleic Acids Res.,11, 1645–1655

    PubMed  Google Scholar 

  • Gupta, S.C., Reese, C.P. and Hastings, J.W. (1985)Arch. Microbiol.,143, 325–329

    Google Scholar 

  • Hastings, J.W. and Nealson, K.H. (1977)Annu. Rev. Microbiol.,31, 549–595

    PubMed  Google Scholar 

  • Karp, M.T., Meyer, P., Suominen, A.I. and Mäntsälä, P.I. (1987a) InBioluminescence and Chemiluminescence, New Perspectives, J. Schölmerich et al., eds., New York: Wiley & Sons, pp. 385–388

    Google Scholar 

  • Karp, M.T., Meyer, P., Lähde, M., Lahti, R. and Mäntsälä, P.I. (1987b) In:Bioluminescence and Chemiluminescence. New Perspectives, J. Schölmerichet al., eds., New York: Wiley & Sons, pp. 361–364.

    Google Scholar 

  • Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982)Molecular Cloning, A. Laboratory Manual, New York: Cold Spring Harbor Laboratory

    Google Scholar 

  • Miyamoto, C., Byers, D., Graham, A.F. and Meighen, E.A. (1987)J. Bacteriol.,169, 247–253

    PubMed  Google Scholar 

  • Reeve, C.A. and Baldwin, T.O. (1982)J. Biol. Chem.,257, 1037–1043

    PubMed  Google Scholar 

  • Vieira, J. and Messing, J. (1982)Gene,19 259–268

    PubMed  Google Scholar 

  • Yanisch-Perron, C., Vieira, J. and Messing, J. (1985)Gene,33 103–109

    PubMed  Google Scholar 

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Korpela, M., Karp, M. Stable-light producingEscherichia coli . Biotechnol Lett 10, 383–388 (1988). https://doi.org/10.1007/BF01087433

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  • DOI: https://doi.org/10.1007/BF01087433

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