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Chemical modification of lipase with ferromagnetic modifier — A ferromagnetic-modified lipase

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Summary

A ferromagnetic modifier was prepared by reacting ferrous(Fe2+)- and ferric(Fe3+)-ions with polyethylene glycol having two carboxyl groups (MW:2000) at pH 8.0–8.5. Lipase fromPseudomonas fragi 22–39B was coupled with the modifier using water-soluble carbodiimide. The modified lipase, which was dispersed into buffered solutions in the size range of 30–70 nm, exerted the hydrolytic activity of 8.0 U/mg. In a magnetic field of 250 Oe, the ferromagnetic-modified lipase was readily recovered from the colloidal solution.

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Authors and Affiliations

  1. Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguroku, Tokyo 152, Japan

    Yutaka Tamaura, Katsunobu Takahashi, Yoh Kodera, Yuji Saito & Yuji Inada

Authors
  1. Yutaka Tamaura
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  2. Katsunobu Takahashi
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  3. Yoh Kodera
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  4. Yuji Saito
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  5. Yuji Inada
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Tamaura, Y., Takahashi, K., Kodera, Y. et al. Chemical modification of lipase with ferromagnetic modifier — A ferromagnetic-modified lipase. Biotechnol Lett 8, 877–880 (1986). https://doi.org/10.1007/BF01078651

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  • DOI: https://doi.org/10.1007/BF01078651

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