Summary
Clostripain was used for peptide bond synthesis with 18 carbobenzoxy-amino acid methyl esters as substrate and PRO-NH2 as nucleophile. Dipeptide bond synthesis was observed only for Cbz-L-ARG-OMe (72%) and Cbz-L-LYS-OMe (34%). With the exception of D-PRO-NH2, nucleophile stereospecificity is not restricted to the L isomer. D- and L-forms of PHE and TRP amides gave the same product yields.
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Fortier, G., MacKenzie, S.L. Substrate- and stereo-specificities in clostripain-catalyzed peptide synthesis. Biotechnol Lett 8, 873–876 (1986). https://doi.org/10.1007/BF01078650
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DOI: https://doi.org/10.1007/BF01078650