Summary
A nuclear polyhedrosis virus ofHyphantria cunea replicated successfully in theTrichoplusia ni cell line. Restriction endonuclease analysis of the viral DNA obtained from infected cell culture showed the same general homogeneity as that from virus isolated from diseased host larvae. Electron microscopic observations showed that the occluded virus from cell culture consists of rod-like nucleocapsids (31×320 nm) enveloped in aggregates and embedded in polyhedral inclusion bodies from 0.6 to 2.5 μm in diameter.
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References
Boucias, D. G. and Nordin, G. L. (1977)J. Invertebr. Pathol. 30, 68–75.
oucias, D. G. and Nordin, G. L. (1980)J. Invertebr. Pathol. 36, 264–272.
Cibulsky, R. I. and Harper, J. D., and Gudauskas, R. T. (1977)J. Invertebr. Pathol. 29, 182–191.
Hink, W. F. and Strauss, E. (1976) In “Invertebrate Tissue Culture, Applications in Medicine, Biology and Agriculture” (Kostak, E. and Maramorosch, K. ed.) 369–374, Academic Press, New York, N.Y.
Ignoff, C. M. and Hink, W. F. (1971) In “Microbial Control of Insects and Mites (Burges, H. D. and Hussey, N. W. ed.) 541–580. Academic Press, New York, N.Y.
Ignoffo, C. M., Shapiro, M., and Hink, W. F. (1971)J. Invertebr. Pathol. 18, 131–134.
Knudson, D. L. and Harrap, K. A. (1976)J. Virol. 17, 254–268.
Lee, H. H. and Miller, L. K. (1978)J. Virol. 27, 754–767.
McClintock, J. T., Dougherty, E. M., and Weiner, R. M. (1986)J. Virol. 57, 197–204.
Miller, L. K. and Dawes, K. P. (1978)Appl. Environ. Microbiol. 35, 411–421.
Pritchett, D. W., Young, S. Y., and Yearian, W. C. (1980).J. Georgia Entomol. Soc. 15, 332–336.
Ramoska, W. A. and Hink, W. F. (1974)J. Invertebr. Pathol. 23, 197–201.
Smith, K. M. (1976) In “Virus-Insect Relationship” (Smith, K. M. ed.) 17–20. Longman, New York, N. Y.
Smith, G. E. and Summers, M. D. (1982)Virol. 123, 393–406.
Sohi, S. S. and Cunningham, J. C. (1972)J. Invertebr. Pathol. 25, 11–17.
Sohi, S. S., Arif, B. M., and Cunningham, J. C. (1984)Intervirol. 21, 50–60.
Sohi, S. S., Percy, J., Cunningham, J. C., and Arif, B. M. (1981)Can. J. Microbiol. 27, 1133–1139.
Suzuki, N. and Kunimi, Y. (1981)Appl. Entomol. Zool. 16, 374–385.
Vail, P. V., Jay, D. L., and Hink, W. F. (1973)J. Invertebr. Pathol. 22 231–237.
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Choe, Y.K., Byun, S.M., Lee, H.H. et al. A nuclear polyhedrosis virus ofHyphantria cunea replicatesin vitro . Biotechnol Lett 8, 853–858 (1986). https://doi.org/10.1007/BF01078644
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DOI: https://doi.org/10.1007/BF01078644