Abstract
Ribonucleic acid (RNA) synthesis was studied in batch and cyclic fed-batch cultures of tylosin-producingStreptomyces fradiae. In batch culture, net RNA synthesis increased with increasing growth rate then decreased as the fermentation progressed. A cyclic response pattern of net RNA synthesis was observed when glucose and glutamate solutions were cyclically fed to the batch culture; RNA synthesis rate increased sharply to a maximum level following the feeding cycles then decreased to lower values between the maxima. Data obtained from an extended cyclic fed-batch culture showed that cyclic feeding of the key nutrients allowed RNA and tylosin syntheses to continue during a period when such activities had ceased in the control batch culture.
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Vu-Trong, K., Gray, P.P. Patterns of RNA synthesis in batch and cyclic fed-batch cultures of tylosin-producingStreptomyces fradiae . Biotechnol Lett 8, 849–852 (1986). https://doi.org/10.1007/BF01078643
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DOI: https://doi.org/10.1007/BF01078643