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Signal transduction through the cAMP-dependent protein kinase

  • Protein Phosphorylation in Signal Transduction
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Abstract

Temporal cellular events responsible for hormonal activation of responses mediated by the cAMP-dependent protein kinase (PKA) have been studied in living cells. By selectively perturbing molecular function of Gs, the catalytic subunit of PKA (C), or the nuclear factor CREB, in cells through microinjection of inhibitory agents specific for these molecules or activated forms of these molecules, we have obtained evidence for a requirement for the function of each of these molecules in the hormonal stimulation of cAMP-regulated genes. Moreover, by introducing fluorescently labeled PKA subunits into these cells as molecular tracers, or by immunofluorescence of C subunit, we have observed biological translocation of C subunit from the cytoplasm to the nucleus during transcriptional activation and a quenching of this by the inhibitor molecule, PKI. The implications of these cellular and molecular events in the signal transduction of hormonal responses are discussed.

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Authors and Affiliations

  1. Department of Medicine, University of California at San Diego, 92093-0636, La Jolla, CA, USA

    Judy L. Meinkoth & James R. Feramisco

  2. Department of Pharmacology, University of California at San Diego, 92093-0636, La Jolla, CA, USA

    Arthur S. Alberts & James R. Feramisco

  3. Department of Chemistry, University of California at San Diego, 92093-0636, La Jolla, CA, USA

    Wei Went, Dominic Fantozzi & Susan S. Taylor

  4. Cancer Center, University of California at San Diego, 92093-0636, La Jolla, CA, USA

    Judy L. Meinkoth, Susan S. Taylor & James R. Feramisco

  5. The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, 92037, La Jolla, CA, USA

    Masatoshi Hagiwara & Marc Montminy

Authors
  1. Judy L. Meinkoth
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  2. Arthur S. Alberts
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  3. Wei Went
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  4. Dominic Fantozzi
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  5. Susan S. Taylor
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  6. Masatoshi Hagiwara
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  7. Marc Montminy
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  8. James R. Feramisco
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Meinkoth, J.L., Alberts, A.S., Went, W. et al. Signal transduction through the cAMP-dependent protein kinase. Mol Cell Biochem 127, 179–186 (1993). https://doi.org/10.1007/BF01076769

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