Skip to main content
Log in

Inhibition of human chymotrypsin by dried egg white

  • Original Articles
  • Published:
The American Journal of Digestive Diseases Aims and scope Submit manuscript

Abstract

Native egg white inhibits trypsin and chymotrypsin of animal origin. The present studies demonstrate that egg white, either raw or spray-dried, is a relatively weak inhibitor of human trypsin but does inhibit human chymotrypsin. Egg white was also shown to significantly inhibit the in vitro digestion of casein by human pancreatic juice. These findings may be important in the nutrition of patients with impaired digestive functions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Delezenne C, Pozerski E: Action empêchante de “l'ovalbumine crue” sur la digestion tryptique de “l'ovalbumine coagulée.” Compt Rend Soc Biol 55:935–937, 1903

    Google Scholar 

  2. Lineweaver H, Murray CW: Identification of the trypsin inhibitor of egg white with ovomucoid. J Biol Chem 171:565–581, 1947

    Google Scholar 

  3. Matsushima K: An undescribed trypsin inhibitor in egg white. Science 127:1178–1179, 1958

    Google Scholar 

  4. Rhodes MB, Bennett N, Feeney RE: The trypsin and chymotrypsin inhibitors from avian egg whites. J Biol Chem 235:1686–1693, 1960

    Google Scholar 

  5. Tomimatsu Y, Clary JJ, Bartulovich JJ: Physical characterization of ovoinhibitor, a trypsin and chymotrypsin inhibitor from chicken egg white. Arch Biochem Biophys 115:536–544, 1966

    Google Scholar 

  6. Feeney RE, Stevens FC, Osuga DT: The specificities of chicken ovomucoid and ovoinhibitor. J Biol Chem 238:1415–1418, 1963

    Google Scholar 

  7. Davis JG, Zahnley JC, Donovan JW: Separation and characterization of the ovoinhibitors from chicken egg white. Biochemistry 8:2044–2053, 1969

    Google Scholar 

  8. Buck FF, Bier M, Nord FF: Some properties of human trypsin. Arch Biochem Biophys 98:528–530, 1962

    Google Scholar 

  9. Kunitz M: Purification and concentration of enterokinase. J Gen Physiol 22:447–450, 1939

    Google Scholar 

  10. Schwert GW, Takenaka Y: A spectrophotometric determination of trypsin and chymotrypsin. Biochim Biophys Acta 16:570–575, 1955

    Google Scholar 

  11. Imondi AR, Stradley RP, Butler ER, Wolgemuth RL: A method for the determination of chymotrypsin in crude biological materials. Anal Biochem 54:199–204, 1973

    Google Scholar 

  12. Imondi AR, Stradley RP, Wolgemuth RL: Synthetic peptides in the diagnosis of exocrine pancreatic insufficiency in animals. Gut 13:726–731, 1972

    Google Scholar 

  13. Smith HW, Finkelstein N, Aliminosa L, Crawford B, Graber M: The renal clearances of substituted hippuric acid derivatives and other aromatic acids in dog and man. J Clin Invest 24:388–404, 1945

    Google Scholar 

  14. Wu FC, Laskowski M: Action of the naturally occurring trypsin inhibitors against chymotrypsins α and β. J Biol Chem 213:609–619, 1955

    Google Scholar 

  15. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951

    Google Scholar 

  16. Allison JB: Nitrogen balance and the nutritive value of proteins. JAMA 164:283–289, 1957

    Google Scholar 

  17. Calloway DH, Margen S: Variation in endogenous nitrogen excretion and dietary nitrogen utilization as determinants of human protein requirement. J Nutr 101:205–216, 1971

    Google Scholar 

  18. Haurowitz F, Tunca M, Schwerin P, Göksu V: The action of trypsin on native and denatured proteins. J Biol Chem 157:621–625, 1945

    Google Scholar 

  19. Morgan AF, Hunt CN, Arnrich L, Lewis E: Evaluation of five partially purified proteins by nitrogen balance in mature dogs, including a study of the anti-tryptic activity of egg white. J Nutr 43:63–75, 1951

    Google Scholar 

  20. Allison JB, Anderson JA, White JU: Evaluation of the nutritional value of proteins with normal and protein-deficient dogs. Trans Am Assoc Cereal Chem 7:24–33, 1949

    Google Scholar 

  21. Baugh CM, Malone JH, Butterworth CE: Human biotin deficiency: a case history of biotin deficiency induced by raw egg consumption in a cirrhotic patient. Am J Clin Nutr 21:173–182, 1968

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Imondi, A.R., Ferguson, J.L. & Wolgemuth, R.L. Inhibition of human chymotrypsin by dried egg white. Digest Dis Sci 18, 1051–1054 (1973). https://doi.org/10.1007/BF01076520

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01076520

Keywords

Navigation