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Expression and localization of intestinal 15 kDa protein in the rat

  • Cellular Fatty Acid-Binding Proteins II
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Abstract

Rat intestinal 15 kDa protein (I-15P) is highly homologous to porcine gastrotropin. We studied the occurrence, distribution and subcellular localization of I-15P in the entire rat body, using the immunocytochemistry to localize protein andin situ hybridization to localize mRNA. Both techniques demonstrated the expression of I-15P in the enterocytes of ileum, luteal cells of ovary and a subpopulation of steroid-endocrine cells of adrenal gland. Immuno-electron microscopy further demonstrated that I-15P is localized in both the cytoplasmic and nuclear matrix regions of these cells. The present results suggest roles of I-15P not only in the transport of bile salts but also in the metabolisms of certain steroid hormones.

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Authors and Affiliations

  1. Department of Anatomy, School of Medicine, Kanazawa University, Kanazawa, 920, Japan

    Shoichi Iseki & Osamu Amano

  2. Department of Biochemistry, Niigata University School of Medicine, Niigata, 951, Japan

    Tatsuo Kanda, Hiroshi Fujii & Teruo Ono

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  1. Shoichi Iseki
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  2. Osamu Amano
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  3. Tatsuo Kanda
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  4. Hiroshi Fujii
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  5. Teruo Ono
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Iseki, S., Amano, O., Kanda, T. et al. Expression and localization of intestinal 15 kDa protein in the rat. Mol Cell Biochem 123, 113–120 (1993). https://doi.org/10.1007/BF01076482

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