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Changes in UsnRNA biosynthesis during rat liver regeneration

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Abstract

Partial hepatectomy (P.H.) induces a partially synchronized growth response of liver under normal regulation of growth. In this phase changes in cellular morphology, radial distribution pattern of cells and other biological as well as major biochemical changes are well documented [24]. Here, we have shown that the cellular content of UsnRNAs altered during this proliferative phase as well. The level of spliceosomal UsnRNAs (U1, U2, U4–U6) gradually decreased by 30–50% upto 48 hrs of P.H. followed by gradual increase to reach the normal level within one month of P.H. the U3 snRNA level on the other hand, was nearly equal to that in normal liver at 48 hrs of P.H. but in 24 and 72 hrs of P.H. its level was high (4 fold) in contrast to that in other UsnRNAs. Thus, it is clear from our data that the level of all the six UsnRNAs decreased during 48 hrs of P.H. compared to that after first 24 hrs. This has been correlated in the kinetics of UsnRNAs' synthesis (in terms of labelling) in isolated hepatocytes, where the rate of labelling of all the six UsnRNAs increased 20–30% in 24 hrs regenerating hepatocytes (R.H.) followed by sharp decrease by 30–50% within next 24 hrs, compared to that in the normal hepatocytes. But from 72 hrs onwards in R.H. the rate of labelling of all the six UsnRNAs again increased by 30–50% (compared to that in normal hepatocytes) followed by decrease of their labelling-rate to reach the normal level in R.H. within one month of P.H. Thus, it may be concluded that the changes in UsnRNAs' level during the proliferative phase of liver regeneration may be either due to the alteration in the rate of synthesis (in terms of labelling) or along with it differential turn over rate; this phenomenon may have some consequences with the regenerative process of liver.

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Authors and Affiliations

  1. Department of Biochemistry, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Calcutta, India

    Rabindranath Ray, Chinmay K. Panda, Bimal K. Chakraborty & Jayashree Roychoudhury

  2. Department of Biophysics, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Calcutta, India

    Sheela Mukherji

  3. Molecular Immunology laboratory, Division of Gastroentrology and Hepatology, University of Medicine and Dentistry of New Jersey, NJ, USA

    Kanakendu Chaudhury

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  1. Rabindranath Ray
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  5. Kanakendu Chaudhury
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Ray, R., Panda, C.K., Chakraborty, B.K. et al. Changes in UsnRNA biosynthesis during rat liver regeneration. Mol Cell Biochem 131, 67–73 (1994). https://doi.org/10.1007/BF01075726

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