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Nerve impulse-induced release of endogenous noradrenaline and adrenaline from the perfused cod spleen

Journal of Comparative Physiology B volume 160, pages 401–406 (1990)Cite this article

Summary

Release of endogenous catecholamines (CA) by electrical nerve stimulation (NS) was studied in the isolated perfused spleen of the Atlantic cod,Gadus morhua. An HPLC-system for the analysis of endogenously released CA is described. Cocaine (COC) was used to block neuronal re-uptake of endogenous CA released by NS. Splanchnic NS at frequencies of 1–40 Hz for 20 s resulted in release of noradrenaline (NA) and adrenaline (A) with a maximal total overflow at 20 Hz for both amines. The release of CA was frequency-dependent. COC (0.1 mmol·l-1) increased NS-evoked (40 Hz) overflow of NA and A by 4.8 and 2.2 times, respectively, and reduced the overflow of dihydroxyphenylglycol (DOPEG) to spontaneous efflux levels or less. It can be concluded that the HPLC-technique used was adequate for measurement of NS-evoked release of endogenous CA and DOPEG from the isolated perfused cod spleen, and the model presented can therefore be used when studying adrenergic mechanisms in fish spleen.

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Abbreviations

A :

adrenaline

CA :

catecholamines

COC :

cocaine

DA :

dopamine

DHBA :

3,4-dihydroybenzylamine hydrobromide

DOPAC :

3,4-dihydroxyphenylacetic acid

DOPEG :

3,4-dihydroxyphenylglycol

l-DOPA :

3,4-dihydroxyphenylalanine

NA :

noradrenaline

NS :

nerve stimulation

PCA :

perchloric acid

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Affiliations

  1. Comparative Neuroscience Unit, Department of Zoophysiology, University of Göteborg, P.O.B. 250 59, S-400 31, Göteborg, Sweden

    F. Ehrenström

  2. Hassle AB, Department for Drug Delivery Research, Kärragatan 5, S-431 83, Mölndal, Sweden

    A. -L. Ungell

Authors
  1. F. Ehrenström
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  2. A. -L. Ungell
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Ehrenström, F., Ungell, A.L. Nerve impulse-induced release of endogenous noradrenaline and adrenaline from the perfused cod spleen. J Comp Physiol B 160, 401–406 (1990). https://doi.org/10.1007/BF01075671

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  • DOI: https://doi.org/10.1007/BF01075671

Key words

  • Endogenous catecholamines
  • High performance liquid chromatography
  • Stimulation-evoked release
  • Spleen
  • Teleost