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Leukocyte migration test in Crohn's disease, ulcerative colitis, and ankylosing spondylitis using Crohn's colon homogenate, mitochondrial, and microsomal fractions

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Abstract

Leukocytes from 33 patients with Crohn's disease, 20 patients with ulcerative colitis, and 20 patients with ankylosing spondylitis were tested for evidence of abnormal migration in the presence of preparations of colon from a patient with Crohn's disease. None of the patients was on treatment with immunosuppressive drugs. The test was also performed with leucocytes from 12 healthy subjects. Significant alteration was seen in the Crohn's disease group, particularly when the antigen used was mitochondrial or microsomal fraction of colon mucosa, whereas the patients in the ulcerative colitis group showed reactivity only with the whole colon homogenate and not with the subcellular fractions. The ankylosing spondylitis group showed no statistical difference from the normal controls with any of the antigens.

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Authors and Affiliations

  1. Royal United Hospital, Combe Park, BA1 3NG, Bath, England

    M. J. Williams MB, MRCP,  E. R. Richens PhD, K. R. Gough MD, FRCP & R. J. Ancill PhD, MD

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  1. M. J. Williams MB, MRCP
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  2. E. R. Richens PhD
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  3. K. R. Gough MD, FRCP
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  4. R. J. Ancill PhD, MD
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Williams, M.J., Richens, E.R., Gough, K.R. et al. Leukocyte migration test in Crohn's disease, ulcerative colitis, and ankylosing spondylitis using Crohn's colon homogenate, mitochondrial, and microsomal fractions. Digest Dis Sci 20, 425–429 (1975). https://doi.org/10.1007/BF01070786

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  • DOI: https://doi.org/10.1007/BF01070786

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