Abstract
Feeding behavior mutants of the fruit flyDrosophila melanogaster having pointmutations were isolated. The defects were analyzed in order to study the mechanism of feeding behavior. Point-mutations namedfebB (three allele) andfebA (three allele) were located between the X-chromosome markerscho andcv and between the markerscv andv, respectively. ThefebB mutants showed a higher acceptance threshold of the proboscis extension reflex than wild-type flies and showed a normal chemosensory response to the electrophysiological test. The mutant (febB 2) showed a higher starvation tolerance than the wild-type fly, but this was not a cause of the high acceptance threshold. Mosaic analysis was applied to the mutant. Internal tissues were suspected to be the primary focus of the abnormal behavior.
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References
Arora, K., Rodrigues, V., Joshi, S., Shanbhag, S. and Siddiqi, O. (1987). A gene affecting the specificity of the chemosensory neurons ofDrosophila. Nature330:62–63.
Bell, W. J., Tortorici, C., Roggero, R. J., Kipp, L. R., and Tobin T. R. (1985). Sucrose-stimulated searching behaviour ofDrosophila melanogaster.Anim. Behav. 33:436–448.
Bentley, D. (1977). Control of cricket song patterns by descending interneurons.J. Comp. Physiol. 116:19–38.
Benzer, S. (1967). Behavioral mutants ofDrosophila isolated by countercurrent distribution.Proc. Natl. Acad. Sci. USA 58:1112–1119.
Benzer, S. (1971). From the gene to behavior.JAMA 218:1015–1022.
Booker, R., and Quinn, W. G. (1981). Conditioning of leg position in normal and mutantDrosophila.Proc. Natl. Acad. Sci. USA 78:3940–3944.
Chen, A. C., and Friedman, S. (1977). Hormonal regulation of trehalose metabolism in the blowflyPhormia regina.J. Insect Physiol. 23:1223–1232.
Dethier, V. G. (1976).The Hungry Fly, Harvard University Press, Cambridge, Mass.
Falk, R., and Atidia, J. (1975). Mutation affecting taste perception inDrosophila melanogaster.Nature (London) 254:325–326.
Fujishiro, N., and Kijima, H. (1988) Difference of median acceptance threshold in proboscis extension reflex between male and female inDrosophila melanogaster.J. Insect Physiol. 34:75–78.
Fujishiro, N., Kijima, H., and Morita, H. (1984). Impulse frequency and action potential amplitude in labellar chemosensory neurones ofDrosophila melanogaster.J. Insect Physiol 30:317–325.
Gelperin, A. (1966). Investigations of a foregut receptor essential to taste threshold regulation in the blowfly.J. Insect Physiol. 12:829–841.
Gelperin, A. (1971). Abdominal sensory neurons providing negative feedback to the feeding behavior of the blowfly.Z. vergl. Physiol. 72:17–31.
Hirakawa, Y., and Kijima, H. (1980). Behavioral analysis of glutathione receptor of Hydra.J. Comp. Physiol. 137:73–81.
Hodgson, E. S., Lettvin, J. Y., and Roeder, K. D. (1955). Physiology of a primary chemoreceptor unit. Science122:417–418.
Horridge, G. A. (1962). Learning of leg position by the ventral nerve cord in headless insects.Proc. Roy. Soc. Lond. B157:33–52.
Hotta, Y., and Benzer, S. (1970). Genetic dissection of theDrosophila nervous system by means of mosaics.Proc. Natl. Acad. Sci. USA 67:1156–1163.
Hotta, Y., and Benzer, S. (1972). Mapping of behavior inDrosophila mosaics.Nature 240: 527–535.
Isono, A., and Kikuchi, T. (1974). Autosomal recessive mutation in sugar response ofDrosophila.Nature 248:243–244.
Kankel, D. R., and Hall, J. C. (1976). Fate mapping of nervous system and other internal tissues in genetic mosaics ofDrosophila melanogaster.Dev. Biol. 48:1–24.
Lewis, E. B., and Bacher, F. (1968). Method of feeding ethyl methane sulfonate (EMS) toDrosophila males.Dros. Inform. Serv. 43:193.
Lindsley, D. L., and Grell, E. H. (1968). Genetic variations ofDrosophila melanogaster. Carnegie Inst. Publ. No. 627, Washington, D.C.
Miyakawa, Y., Fujishiro, N., Kijima, H., and Morita, H. (1980). Differences in feeding response to sugars between adults and larvae inDrosophila melanogaster.J. Insect Physiol. 26:685–688.
Rodrigues, V., and Siddiqi, O. (1978). Genetic analysis of chemosensory pathway.Proc. Ind. Acad. Sci. 81B:147–160.
Rodrigues, V., and Siddiqi, O. (1981). A gustatory mutant ofDrosophila defective in pyranose receptors.Mol. Gen. Genet. 181:406–408.
Schilcher, F. von, and Hall, J. C. (1979). Neural topography of courtship song in sex mosaics ofDrosophila melanogaster.J. Comp. Physiol. 129:85–95.
Shiraishi, A., Yano, T., and Nakashima, M. (1979) Effects of interruption of the recurrent nerve on the feeding behavior of the blowfly,Phormia regina, M.Integrat. Control Funct. Brain 2:341–342.
Tanimura, T., and Shimada, I. (1981). Multiple receptor proteins for sweet taste inDrosophila discriminated by papain treatment.J. Comp. Physiol. 141:265–269.
Tompkins, L., Cardosa, M. J., White, F. V., and Sanders, T. G. (1979). Isolation and analysis of chemosensory behavior mutants inDrosophila melanogaster.Proc. Natl. Acad. Sci. USA 76:884–887.
Wieczorek, H., and Wolff, G. (1989). The labellar sugar receptor ofDrosophila.J. Comp. Physiol. A 164:825–834.
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Fujishiro, N., Kijima, H. & Miyakawa, Y. Isolation and characterization of feeding behavior mutants inDrosophila melanogaster . Behav Genet 20, 437–451 (1990). https://doi.org/10.1007/BF01065568
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DOI: https://doi.org/10.1007/BF01065568