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Inhalation exposure to 1,3-dichloropropene in the Dutch flower-bulb culture. Part II. Biological monitoring by measurement of urinary excretion of two mercapturic acid metabolites

Archives of Environmental Contamination and Toxicology volume 20, pages 6–12 (1991)Cite this article

Abstract

A biological monitoring study was carried out in the Dutch flower-bulb culture to determine the relationship between respiratory occupational exposure to Z- and E-1,3-dichloropropene (Z- and E-DCP) and urinary excretion of two mercapturic acid metabolites, N-acetyl-S-(Z- and E-3-chloropropenyl-2)-L-cysteine (Z- and E-DCP-MA). Urinary excretion of Z- and E-DCP-MA, either based on excretion rates or on creatinine excretion, followed first order elimination kinetics after exposure. Urinary half-lives of elimination were 5.0 ± 1.2 hr for Z-DCP-MA and 4.7 ± 1.3 hr for E-DCP-MA and were not statistically significantly different. Calculated coefficients of variation indicated that the half-lives of elimination of Z- and E-DCP-MA were quite consistent inter- and intra-individually.

Strong correlations (r ⩾ 0.93) were observed between respiratory 8-hr time weighted average (TWA) exposure to Z-and E-DCP and complete cumulative urinary excretion of Z- and E-DCP-MA. Z-DCP yielded three times more mercapturic acid than E-DCP, probably due to differences in metabolism. Z- and E-DCP were excreted 45 and 14% as their respective mercapturic acid metabolites.

A respiratory 8-hr TWA exposure to the Dutch occupational exposure limit of 5 mg · m−3 DCP would result in a complete cumulative excretion of 14.4 mg (95% confidence interval: 11.7–17.0 mg) Z-DCP-MA and 3.2 mg (95% confidence interval: 2.3–4.1 mg) E-DCP-MA.

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Affiliations

  1. Department of Pharmacochemistry, Molecular Toxicology, Free University, De Boelelaan 1083, 1081, HV Amsterdam, The Netherlands

    R. T. H. van Welie, P. van Duyn & N. P. E. Vermeulen

  2. Department of Occupational Toxicology, TNO Medical Biological Laboratory, P.O. Box 45, 2280, AA Rijswijk, The Netherlands

    D. H. Brouwer & J. J. van Hemmen

  3. Toxicology Laboratory, University Hospital, P.O. Box 9600, 2300, RC Leiden, The Netherlands

    E. J. Brouwer

Authors
  1. R. T. H. van Welie
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  2. P. van Duyn
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  3. D. H. Brouwer
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  4. J. J. van Hemmen
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  5. E. J. Brouwer
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  6. N. P. E. Vermeulen
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van Welie, R.T.H., van Duyn, P., Brouwer, D.H. et al. Inhalation exposure to 1,3-dichloropropene in the Dutch flower-bulb culture. Part II. Biological monitoring by measurement of urinary excretion of two mercapturic acid metabolites. Arch. Environ. Contam. Toxicol. 20, 6–12 (1991). https://doi.org/10.1007/BF01065321

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  • DOI: https://doi.org/10.1007/BF01065321

Keywords

  • Urinary Excretion
  • Occupational Exposure
  • Biological Monitoring
  • Inhalation Exposure
  • Elimination Kinetic