Summary
In the presence of cytochalasin B (CCB) concentrations from 50 to 200 μg/ml there is a dose-dependant inhibition of insulin release from isolated rat pancreatic islets. Inhibition is unspecific with respect to glucose, leucine, tolbutamide or theophylline and is reversible. Production of14CO2 from uniformly labeled D-glucose is decreased. Islets pretreated with an high (200 μg/ml) or low (10 μg/ml) CCB dose release more insulin in response to a subsequent glucose or leucine stimulus in a CCB free medium compared with controls. The data are compatible with a membrane-directed action of CCB.
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Schauder, P., Frerichs, H. Cytochalasin B: Evidence for a membrane-directed action in B-cells from rat pancreatic islets. Pflugers Arch. 364, 95–98 (1976). https://doi.org/10.1007/BF01062918
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DOI: https://doi.org/10.1007/BF01062918