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Structural and enzymatic properties of cardiac myosin in ischaemic and non-ischaemic regions of the rat myocardium

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Summary

In rats with ligation of the left coronary artery changes in ATPase activity and structure of cardiac myosin in both ischaemic and non-ischaemic zones of the myocardium were followed. In control aninals. ATPase activity and the structure of the myosin molecule in right and left ventricles did not differ. Non-specific factors, such as anaesthesia and thoracotomy, can result in a decrease or an increase in ATPase activity respectively.

One hour after ligation of the left coronary artery ATPase activity increased in the right, non-ischaemic myocardium and there was a significant right-to-left difference. Four hours after ligation, ATPase activity in both ventricles significantly decreased and the right-left difference disappeared. Within 48 h, normal values were found only in the non-ischaemic right ventricle.

Ligation of the left coronary artery results after 48 h in the formation of structural alterations in cardiac myosin, primarily in the left, ischaemic myocardium. These changes are characterised by the formation of myosin aggregates, which have a significantly lower ATPase activity in comparison with monomeric myosin.

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Pelouch, V., Ošťádal, B. & First, T. Structural and enzymatic properties of cardiac myosin in ischaemic and non-ischaemic regions of the rat myocardium. Pflugers Arch. 364, 1–6 (1976). https://doi.org/10.1007/BF01062904

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  • DOI: https://doi.org/10.1007/BF01062904

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