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Comparison of the enzymatic composition of cell-free extracts of non-aflatoxigenicAspergillus parasiticus with respect to late stages of aflatoxin biosynthesis

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Abstract

Cell-free extracts of fungal mycelia of two non-aflatoxigenic isolates ofAspergillus parasiticus (SRRC 163 and SRRC 2043) were examined for enzyme activities involved in the latter stages of aflatoxin biosynthesis. Post-microsomal fractions (105 Kxg supernatant) of both SRRC 163 (ATTC 56774) and SRRC 2043 were able to convert sterigmatocystin (ST) toO-methylsterigmatocystin (OMST); whereas the microsomal (105 Kxg pellet) preparation of only SRRC 163 was able to convert OMST to aflatoxin B1 (AFB1). A mixture of the microsomal fraction of SRRC 163 and post-microsomal fraction of SRRC 2043 converted ST to AFB1. Electrophoretic protein separations of the microsomal fractions of the twoA. parasiticus isolates on non-denaturing or denaturing polyacrylamide gel electrophoresis (PAGE) provided equivalent protein profiles.

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Authors and Affiliations

  1. U. S. Department of Agriculture, ARS, Southern Regional Research Center, Robert E. Lee Blvd, P. O. Box 19687 1100, 70179, New Orleans, Louisiana, USA

    Deepak Bhatnagar, Thomas E. Cleveland & Alan R. Lax

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  1. Deepak Bhatnagar
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  2. Thomas E. Cleveland
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  3. Alan R. Lax
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Bhatnagar, D., Cleveland, T.E. & Lax, A.R. Comparison of the enzymatic composition of cell-free extracts of non-aflatoxigenicAspergillus parasiticus with respect to late stages of aflatoxin biosynthesis. Arch. Environ. Contam. Toxicol. 18, 434–438 (1989). https://doi.org/10.1007/BF01062370

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