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Process of penicillin G hydrolysis catalyzed by penicillin acylase immobilized on acylic carrier

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Abstract

The usefulness of penicillin acylase immobilized onto butyl acrylate — ethyl glycol dimethacrylate (called in this paper acrylic carrier) in penicillin G hydrolysis performed in a stirred tank reactor is shown. The enzyme-acrylic carrier preparation does not deteriorate its own properties in the mixing condition of slurry reactor. The experiments were carried out in a batch and a continuous stirred tank reactor as well as continuous stirred tank reactors in series. It was found to be a satisfactory agreement between experimental and predicted results. It also indicated the optimal substrate concentration range which provides the most effective enzyme operation. A superiority of the three reactors in series over the batch reactor is shown.

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Abbreviations

CE g/m3 :

equivalent enzyme concentration

CSO mol/m3 :

initial penicillin G concentration

KA mol/m3 :

substrate affinity constant

KiS mol/m3 :

substrate inhibitory constant

KiP mol/m3 :

PhAA inhibitory constant

KiQ mol/m3 :

6-APA inhibitory constant

k3 mol/g min:

constant rate of dissotiation of the active complex

r mol/m3 :

rate of reaction

t min.:

reaction time

tj min.:

maintenance time

α:

degree of conversion

ΘB, ΘF :

dimensionless time

τ min.:

residence time

PA:

penicillin acylase

PG:

penicillin G

PhAA:

phenylacetic acid

6-APA:

6-aminopenicillanic acid

References

  1. Shewale, J. G.; Sivaraman, H.: Penicillin acylase: Enzyme production and its application in the manufacture of 6-APA. Bioprocess Biochem. Aug (1989) 146–154

  2. Ospina, S. S.; Lopez-Munguia, A.; Gonzales, R. L.; Quintero, R.: Characterization and use of a penicillin acylase biocatalyst. J. Chem. Tech. Biotechnol. 53 (1992) 205–214

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  3. Bryjak, J.; Trochimczuk, A.; Noworyta, A.: Effect of polymer matrix on penicillin acylase immobilization on copolymers of butyl acrylate and ethylene glycol dimethacrylate. J. Chem. Technol. Biotechnol. 57, 1 (1993) 73–78

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  4. Bryjak, J.; Noworyta, A.: Immobilization of penicillin acylase on copolymer of butyl acrylate and ethylene glycol dimethacrylate. J. Chem. Technol. Biotechnol. 57, 1 (1993) 79–86

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  5. Bryjak, J.; Noworyta, A.: Kinetic behaviour of penicillin acylase immobilized on acrylic carrier. Bioprocess Eng. 9, 1 (1993) 37–42

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Authors and Affiliations

  1. Institute of Chemical Engineering, Technical University of Wroclaw, Norwida 6, 50-373, Wroclaw, Poland

    A. Noworyta & J. Bryjak

Authors
  1. A. Noworyta
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  2. J. Bryjak
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Noworyta, A., Bryjak, J. Process of penicillin G hydrolysis catalyzed by penicillin acylase immobilized on acylic carrier. Bioprocess Engineering 9, 271–275 (1993). https://doi.org/10.1007/BF01061533

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  • DOI: https://doi.org/10.1007/BF01061533

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