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Growth and cytochrome P-450 of yeasts subjected to various Foreign chemicals

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Abstract

Fourteen chemicals, including phenobarbital, two polycyclic aromatic hydrocarbons, one organophosphate, and ten chlorinated compounds were chosen to study the inducibility of cytochrome P-450 in the yeastSaccharomyces cerevisiae. Kepone®, hexachlorophene, 2,4,6-trichlorophenol, and 2,6-dichlorophenol were toxic toS. cerevisiae, inhibiting its growth even at low concentrations. Clophen A60 ® (a PCB-mixture) and lindane increased the concentration of cytochrome P-450 by 32% and 50%, respectively. Clophen A60 enhanced the concentration of this cytochrome also in the yeastHansenula anomala, but not inPichia fermentans, Saccharomyces chevalieri, andTorulopsis glabrata. Lindane increased P-450 concentration inT. glabrata but had no effect on this hemoprotein inKluyveromyces fragilis. In contrast to cultured hepatocytes, these foreign compounds were poor inducers or non-inducers of cytochrome P-450 in the yeasts studied.

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Authors and Affiliations

  1. Department of Biochemistry, University of Kuopio, SF-70101, Kuopio 10, Finland

    Sirpa O. Kärenlampi & Eine Marin

  2. Department of Physiology, University of Kuopio, SF-70101, Kuopio 10, Finland

    Osmo O. P. Hänninen

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  1. Sirpa O. Kärenlampi
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  2. Eine Marin
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  3. Osmo O. P. Hänninen
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Kärenlampi, S.O., Marin, E. & Hänninen, O.O.P. Growth and cytochrome P-450 of yeasts subjected to various Foreign chemicals. Arch. Environ. Contam. Toxicol. 11, 693–698 (1982). https://doi.org/10.1007/BF01059156

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  • DOI: https://doi.org/10.1007/BF01059156

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