Expression of results from growth inhibition toxicity tests with algae

  • Niels Nyholm
Article

Abstract

Results from six growth inhibition toxicity tests with the freshwater green algaeSelenastrum capricornutum andScenedesmus subspicatus have been analyzed, using both biomass measurements and estimates of the specific growth rate to quantify the toxic response. For exponentially growing cultures, biomass derived effective concentrations (EC's) decrease with time, but reach asymptotic values in the course of some days. The final (asymptotic) values are smaller than EC figures derived from growth rates, and the differences are greater, the smaller the slope of the dose response curve. EC50 values differed by up to a factor of 5. The examples presented are in agreement with a previously presented mathematical analysis. The author recommends that standard test protocols for routine testing should prescribe the use of some appropriate measure of the specific growth rate-rather than the biomass-as the response variable.

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References

  1. Adams N, Goulding KH, Dobbs AJ (1985) Toxicity of eight watersoluble organic chemicals toSelenastrum capricornutum: A study of methods for calculating toxic values using different growth parameters. Arch Environ Contam Toxicol 14:333–345Google Scholar
  2. Bahner LH, Oglesby JL (1982) Models for predicting bioaccumulation and ecosystem effects of Kepone® and other materials. In Conway RA (ed) Environmental Risk Analysis for Chemicals, Van Nostrand Reinhold 461–473Google Scholar
  3. Blanck H (1983) On the impact of Long-Chained Aliphatic Amines on Photosynthesis and Algal Growth in Ecotoxicological Test Systems. Doctoral Dissertation. Department of Plant Physiology, University of Gothenburg, Gothenburg, SwedenGoogle Scholar
  4. Commission of the European Communities (EEC) (1985) EEC Directive 79:831, Annex V, Part C: Methods for determination of ecotoxicity-level 1. C(L1)1: Algal Inhibition Test, DGXI/723/81, Brussels, BelgiumGoogle Scholar
  5. Hanstveit AO (1982) Evaluation of the results of the second ISO-interlaboratory study with an algal toxicity test. Netherlands Organization for Applied Scientific Research, TNO, prepared for ISO/TC 147/SC5/WG 5. Nederlands Normalisatie Instituut. Delft, The NetherlandsGoogle Scholar
  6. International Standards Organization (ISO) (1982) Draft method Algal Growth Inhibition Test. ISO/TC 147/SC 5/WG 5 N28 Nederlands Normalisatie-instituut. Delft, The NetherlandsGoogle Scholar
  7. (1987) Water quality, Algal Growth Inhibition Test. Draft International Standard ISO/DIS 8692Google Scholar
  8. Källqvist T (1978) Noen erfaringer av alge-toksisitets tester ved NIVA. In Toxicitetstester, Fjortonde Nordiske Symposiet om Vattenforskning. Nordic Cooperative Organization for Applied Research, Secretariate of Environmental Sciences, publication 1978:2 Helsinki, Finland, 147–166Google Scholar
  9. Miller WE, Greene JC, Shiroyama T (1976) Use of algal assays to define trace element limitation and heavy metal toxicity. In Proc of the Symposium on Terrestrial and Aquatic Ecological Studies of the Northwest. EWSC Press, Eastern Washington State College, WashingtonGoogle Scholar
  10. — (1978) TheSelenastrum capricornutum Printz Algal Assay Bottle Test. Experimental Design, Application, and Data Interpretation Protocol. EPA-600/9-78-08, US Environmental Protection Agency, Corvallis, Oregon 97330Google Scholar
  11. Nyholm N (1985) Response variable in algal growth inhibition testsbiomass or growth rate? Review Paper. Water Res 19:273–279Google Scholar
  12. Nyholm N, Källqvist T 1989. Methods for growth inhibition toxicity tests with freshwater algae. Environ Toxicol Chem 8:689–703Google Scholar
  13. Organization for Economic Cooperation, and Development (OECD) (1984) Test Guideline 201 (“Alga, Growth Inhibition Test”) Adopted June 7, 1984. OECD, Paris, FranceGoogle Scholar
  14. Statistical Analysis System (1979) SAS User's Guide, 1979 ed, SAS Institute, Raleigh, North Carolina 27605Google Scholar

Copyright information

© Springer-Verlag New York Inc 1990

Authors and Affiliations

  • Niels Nyholm
    • 1
  1. 1.Water Quality InstituteHørsholmDenmark

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