In vivo andIn vitro studies on synergism with anticholinesterase pesticides in the snailLymnaea acuminata

  • D. K. Singh
  • R. A. Agarwal


The synergistic effects of piperonyl butoxide (PB)2, sulfoxide (SU), and dimethyl amino aniline (DAA) used in conjunction with two organophosphate pesticides (phorate and formothion) and two carbamate pesticides (mexacarbate and carbaryl) were examined in terms of the LC50 and acetylcholinesterase (AChE) inhibition in the snailLymnaea acuminata. The three synergists reduced the LC50 of phorate, formothion, mexacarbate, and carbaryl at exposure periods ranging from 24 to 144 hr. PB and SU had strong synergistic action; DAA had little effect; the highest synergistic ratio (70) was found with carbaryl and PB. The synergists enhanced thein vitro inhibition of cholinesterase caused by the pesticides; the synergistic effect onin vitro inhibition, however, was lower, compared to the change in the LC50.


Synergistic Effect Cholinesterase Carbamate Acetylcholinesterase Exposure Period 
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Copyright information

© Springer-Verlag New York Inc. 1983

Authors and Affiliations

  • D. K. Singh
    • 1
  • R. A. Agarwal
    • 1
  1. 1.Department of ZoologyUniversity of GorakhpurGorakhpurIndia

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