Abstract
In L-2071 tissue culture cells, 50 ppm (50 Μg/ml culture medium) each of dieldrin or DDT reduced total cell count and the rate of14C-leucine incorporation into protein and14C-uridine incorporation into total RNAs; this was the result of an increased rate of14C-uridine incorporation into cytoplasmic soluble and 4S RNA cellular fraction. Total amino acid acceptor activity decreased with dieldrin and DDT treatments, which suggests an actual decrease in functional tRNAs. Dieldrin reduced glutamyl- and aspartyl-tRNA activities whereas phenylalanyl-, tyrosyl-, threonyl-, leucyl-, isoleucyl-, seryl-, and prolyl-tRNA activities decreased with both pesticides. Valyl-, lysyl-, glycyl-, alanyl-, histidyl-, and arginyl-tRNA activities were only slightly changed. The effect of dieldrin was generally greater than that of DDT. In addition, there was degradation of 28S and 18S RNAs to fragments isolated with 4S RNAs. Cells treated with 0.5 and 10 ppm levels of dieldrin demonstrated decreased14C-uridine incorporation into 28S RNAs and increased incorporation into 18S and 4S RNAs after 3 hours and 3 to 6 hours, respectively. Incorporation levels returned to near control levels, thereafter, in whole cell preparations, but remained different from controls when nuclear and cytoplasmic fractions were assessed individually.
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References
Ahmed FE, Hart RW, Lewis NJ (1977) Pesticide-induced DNA damage and its repair in cultured human cells. Mutation Res 16:161–166
Antunes-Madeira MC, Madeira VMC (1979) Interaction of insecticides with lipid membranes. Biochem Biophys Acta 550:384–386
Bridges KR, Jones GH (1973) Transfer of ribonucleic acids from mouse plasmacytoma tumors produced k and λ immunoglobulin chains. Biochemistry 12:1208–1212
Bruno GA, Christian JE (1961) Determination of carbon-14 in aqueous bicarbonate solutions by liquid scintillation counting techniques. Anal Chem 33:1216–1218
Buff K, Bründl A (1984) Selective alterations of membrane properties of cultured human liver cells caused by the insecticide DDT. Pestic Biochem Physiol 22:36–42
Burton KA (1956) A study of the conditions of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J 62:315–323
Carpousis A, Christner P, Rosenbloom J (1977) Preferential usage of tRNA isoaccepting species in collagen synthesis. J Biol Chem 252:8023–8026
Chavancy G, Chevallier A, Fournier A, Garel JP (1979) Adaptation of iso-tRNA concentration to mRNA codon frequency in the eukaryotic cell. Biochemie 61:71–78
Chen GS, Siddiqui MAQ (1974) Involvement of glycine transfer ribonucleic acids in development of the posterior silk gland ofBombyx mori. Arch Biochem Biophys 161:109–117
Chung RA, Brown RW, Beacham LH, Huang IL (1966) Amino acid metabolism of different mammalian cell lines. Can J Biochem 44:1145–1157
Chung RA, Huang IL, Brown RW (1967) Studies of DNA, RNA and protein synthesis in HeLa S cells exposed to DDT and dieldrin. J Agric Food Chem 15:497–500
Chung RA, Lin YD, Brown RW (1968) Effect of DDT and dieldrin on RNA and protein synthesis in subcellular fractions of HeLa S cells. J Agric Food Chem 16:298–299
Eagle H (1959) Amino acid metabolism in mammalian cell cultures. Science 130:432–437
Garcia M, Mourelle M (1984) Gamma-glutamyl transpeptidase: A sensitive marker in DDT and toxaphene exposure. J Appl Toxicol 4:246–248
Ghadially FN (1982) Ultrastructural pathology of the cell and matrix, 2nd edition. Butterworths, London, pp 330–335
Griffith J, Duncan RC (1985) Serum organochlorine residues of Florida citrus workers compared to the national health and nutritional examination survey sample. Bull Environ Contam Toxicol 35:411–417
Jones GH (1982) Protein synthesis in bupivacaine (marcaine) treated regenerating skeletal muscle. Muscle and Nerve 5:281–290
— (1983) Acceptor activity, isoacceptor profiles and function in protein synthesis of transfer RNAs from regenerating skeletal muscle. Biochem Biophys Acta 741:333–340
Kelmers AD, Novelli GD, Stulberg MP (1965) Separation of transfer-ribonucleic acids by reverse phase chromatography. J Biol Chem 240:3979–3983
Kleiman L, Woodward Jack J, Cedergren RJ, Dion R (1978) Alterations in lysine transfer RNA during erythroid differentiation of the Friend cell. Nucleic Acids Res 5:851–859
Koch FC, McMeekin T (1924) A new direct Nesslerization micro-kjeldahl method and a modification of the NesslerFolin reagent for ammonia. J Am Chem Soc 46:2066–2069
Larson PF, Hudgson P, Walton JN (1969) Morphological relationship of polyribosomes and myosin filaments in developing and regenerating skeletal muscle. Nature 222:1168–1169
Mejbaum W (1939) Liber die Bestimmung Kleiner Pentosemengen, insbesondere in Derivaten der Adenylsaure, Hoppe-Seyler's. Ztschr F Physiol Chem 258:117–120
Monod G (1985) Egg mortality of Lake Geneva charr (Salvelinus-alpinus L) contaminated by PCB and DDT derivatives. Bull Environ Contam Toxicol 35:531–536
Murakami M, Fukami J (1979) Uptake of benzo[a]pyrene, carbamyi, DDT and parathion in cultured human cells: Re-evaluation. Bull Environ Contam Toxicol 21:478–482
— (1980) Incorporation of labeled pesticides and environmental chemicals into nuclear fractions of cultured human cells. Bull Environ Contam Toxicol 24:27–30
Ortwerth BJ (1971) Isoaccepting transfer ribonucleic acids in specialized mammalian tissues. Biochemistry 10:4190–4197
Ortwerth BJ, Liu LP (1973) Correlation between a specific isoaccepting lysyl transfer ribonucleic acid and cell division in mammalian tissues. Biochemistry 12:3978–3984
Ortwerth BJ, Yonuschot GR, Carlson JV (1973) Properties of tRNALys from various tissues. Biochemistry 12:3985–3991
Peacock AC, Dingman CW (1967) Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochem J 6:1818–1827
Penman S (1966) RNA metabolism in the HeLa cell nucleus. J Mol Biol 17:117–180
Robison AK, Sirbasku DA, Stancel GM (1985) DDT supports the growth of an estrogen-responsive tumor. Toxicol Lett 27:109–113
RupLal and Saxena DM (1980) Effect of DDT on cell population growth, cell division and DNA synthesis inStylonychia notophora (Stokes) Arch Environ Contam Toxicol 9:163–170
Scherrer K, Darnell JE (1962) Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Comm 7:486–490
Steel RG, Torrie GH (1980) Principles and procedures of statistics. McGraw Hill, New York
Tennekes H, van Ravenzwaay B, Kunz HW (1985) Quantitative aspects of enhanced liver tumor formation in CF-1 mice by dieldrin. Carcinogenesis 6:1457–1462
Trump BF, Jesudason ML, Jones RT (1978) Ultrastructural features of diseased cells. In: Trump BF, Jones RT (eds) Diagnostic electron microscopy, Vol 1. John Wiley and Sons, New York, pp 1–88
Tsushimoto G, Chang CC, Trosko JE, Matsumura F (1983) Cytotoxic, mutagenic and cell-cell communication inhibitory properties of DDT, lindane, and chlordane on Chinese hampster cellsin vitro. Arch Environ Contam Toxicol 12:721–730
Volker SAS, Taylor MW (1971) Chromatographic comparison of the transfer ribonucleic acids of rat livers and Morris hepatomas. Biochemistry 10:488–497
WÄrngard L, Flodström S, Ljungquist S, Ahlborg UG (1985) Inhibition of metabolic cooperation in Chinese hamster lung fibroblast cells (V79) in culture by various DDT-analogs. Arch Environ Contam Toxicol 14:541–546
Weisenberg E, Arad I, Graver F, Sahm Z (1985) Polychlorinated biphenyls and organochlorine insecticides in human milk in Israel. Arch Environ Contam Toxicol 14:517–522
Yang WK, Novelli GD (1968) Multiple iso-accepting transfer RNA's in a mouse plasma cell tumor. Proc Natl Acad Sci USA 59:280–315
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Chung, R.A., Williams, C.S. RNA synthesis and aminoacyl transfer RNA activity of L-2071 cells exposed to dieldrin and DDT. Arch. Environ. Contam. Toxicol. 15, 581–588 (1986). https://doi.org/10.1007/BF01056572
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DOI: https://doi.org/10.1007/BF01056572