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Use of Microtox™for assessing copper complexation with organic compounds

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Abstract

Microtox™ bioassay was used for assessing the complexation of copper with organic compounds including humic acids and corn root exudates. The bioassay, based on reduction of bacterial luminescence in the presence of toxicants, consists of determining the toxicity of organo-metallic complexes and thus assessing the bioavailability of copper to the test bacteria. Natural organic compounds reduced the extent of copper toxicity. Thus, Microtox can be used as a rapid tool for assessing the interactions of heavy metals with organic ligands.

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References

  • Babich H, Stotzky G (1980) Environmental factors that influence the toxicity of heavy metals and gaseous pollutants to microorganisms. Crit Rev Microbiol 8:99–145

    PubMed  Google Scholar 

  • Babich H, Stotzky G (1986) Environmental factors affecting the utility of microbial assays for the toxicity and mutagenicity of chemical pollutants. In: Dutka BJ, Bitton G (eds) Toxicity testing using microorganisms, vol 2. CRC Press, Boca Raton, FL, pp 9–42

    Google Scholar 

  • Beckman Instruments (1982) Microtox system operating manual. No. 015-555-879. Carlsbad, CA

  • Bitton G (1983) Bacterial and biochemical tests for assessing chemical toxicity in the aquatic environment: A review. CRC Crit Rev Environ Control 13:51–63

    Google Scholar 

  • Bitton G, Dutka BJ (eds) (1986) Toxicity testing using microorganisms, vol 1. CRC Press, Boca Raton, FL

    Google Scholar 

  • Bizri Y, Cromer M, Scharff JP, Guillet B, Rouiller J (1984) Constantes de stabilité de complexes organo-minérau. Interactions des ions plombeux avec les composés organiques hydrosolubles des eaux gravitaries de podzol. Geochim Cosmochim Acta 48:227–234

    Google Scholar 

  • Bulich AA (1986) Bioluminescence assays. In: Bitton G, Dutka BJ (eds) Toxicity testing using microorganisms, vol 1. CRC Press, Boca Raton, FL, pp 57–74

    Google Scholar 

  • Cortez J, Billes G (1982) Role des ions calcium dans la formation du mucigel de Zea mays. Acta Oecol, Oecol Plant 17:67–78

    Google Scholar 

  • Dutka BJ, Bitton G (eds) (1986) Toxicity testing using microorganisms, vol. 2. CRC Press, Boca Raton, FL

    Google Scholar 

  • Gadd GM, Griffiths AJ (1978) Microorganisms and heavy metal toxicity. Microb Ecol 4:303–331

    Google Scholar 

  • Lepp NW (1981) Effect of heavy metals pollution on plants. Vol 1, Effect of trace metals on plant function. Applied Science Publishers, London

    Google Scholar 

  • McKeague JA, Cheschire MV, Andreux F, Berthelin J (1986) Organo-mineral complexes in relation to pedogenesis. In: Huang PM (ed) Interactions of soil minerals with natural organics and microbes. Soil Sci Soc Am, Madison, WI

    Google Scholar 

  • Mench M, Morel JL, Guckert A (1985) Liaison du cadmium avec la fraction macromoléculaire soluble des exsudats racinaires de mais (Zea mays L.). CR Acad Sci 301:379–382

    Google Scholar 

  • — 1987 Metal binding properties of high molecular weight soluble exudates from maize. Biol Fertil Soils 3:165–169

    Google Scholar 

  • Morel JL, Mench M, Guckert A (1986) Measurement of Pb2+, Cu2+and Cd2+ binding with mucilage exudates from maize (Zea mays L.) roots. Biol Fertil Soils 2:29–34

    Google Scholar 

  • Mortensen JL (1963) Complexing of metal by soil organic matter. Soil Sci Soc Am Proc 27:179–186

    Google Scholar 

  • Reuter JH, Perdue EM (1977) Importance of heavy metal-organic matter interactions in natural waters. Geochem Cosmochim Acta 41:325–334

    Google Scholar 

  • Schnitzer M, Kahn SU (1972) Humic substances in the environment. Marcel Dekker, New York

    Google Scholar 

  • Sposito G, Holtzclaw KM, Levesque-Madore CS (1979) Cupric ion complexation by fulvic acid extracted from sewage sludge-soil mixture. Soil Sci Soc Am J 43:1148–1155

    Google Scholar 

  • Stevenson FJ (1976) Stability constants of Cu2+, Pb2+ and Cd2+ complexes with humic acids. Soil Sci Soc Am J 40:665–672

    Google Scholar 

  • Stevenson FJ, Ardakani MS (1972) Organic matter reactions involving micronutrients in soils. In: Mordvedt JJ, Giordano PM, Lindsay WL (eds) Micronutrients in agriculture. Soil Sci Soc Am, Madison, WI, pp 79–114

    Google Scholar 

  • Stevenson FJ, Fitch A (1981) Reactions with organic matter. In: Loneragan JF, Robson AD, Robson AD and Graham RD (eds) Copper in soils and plants. Academic Press, Australia, pp 69–95

    Google Scholar 

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Author notes

  1. Jean -Louis Morel

    Present address: Ecole Nationale Superieure, d Agronomie et des Industries Alimentaires, Vandoeuvre, France

Authors and Affiliations

  1. Department of Environmental Engineering Sciences, University of Florida, 32611, Gainesville, Fiorida

    Jean -Louis Morel, Gabriel Bitton & Ben Koopman

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  1. Jean -Louis Morel
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  2. Gabriel Bitton
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  3. Ben Koopman
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Morel, J.L., Bitton, G. & Koopman, B. Use of Microtox™for assessing copper complexation with organic compounds. Arch. Environ. Contam. Toxicol. 17, 493–496 (1988). https://doi.org/10.1007/BF01055514

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  • DOI: https://doi.org/10.1007/BF01055514

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