Skip to main content
Log in

Combined use of biochemical indicators to assess sublethal pollution effects on the Gulf killifish (Fundulus grandis)

  • Published:
Archives of Environmental Contamination and Toxicology Aims and scope Submit manuscript

Abstract

Sublethal biochemical markers were used to identify liver enzyme induction in fish from a bayou in Pensacola, Florida. Gulf killifish,Fundulus grandis, from, a nonpolluted site were used in the study which included laboratory-induced fish and their various controls as well as the fish captured in the bayou. The biochemical markers tested were total content of cytochrome P-450, aryl hydrocarbon hydroxylase and ethoxyresorufin-O-deethylase activities, and the specific induction of the LM4bisozyme of the cytochrome P-450 system. The findings suggest that enzyme induction occurred at a sublethal level, indicating the presence of liver cytochrome P-450-inducing substances in the bayou.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ahokas JT, Pelkonen A, and Karki NT (1976) Cytochrome P-450 and drug-induced spectral interactions in the hepatic microsomes of trout,Salmo trutta lacustris. Acta Pharmacol Toxicol 38:440–449

    Google Scholar 

  • Conney AH (1982) Induction of microsomal enzymes by foreign chemicals and carcinogenesis by polycyclic aromatic hydrocarbons: G.H.A. Clowes Memorial lecture. Cancer Res 42:4875–4917

    PubMed  Google Scholar 

  • Department of the Interior, U.S. Geological Survey (1986) Movement and fate of creosote waste in ground water, Pensacola, Florida: US Geological Survey Toxic Waste-Ground-Water Contamination Program. US Geological Survey Water-Supply Paper 2285:1–63. US Government Printing Office, Washington, DC

    Google Scholar 

  • Elcombe CR, Lech JJ (1979) Induction and characterization of hemoprotein(s) P-450 and monooxygenation in rainbow trout (Salmo gairdneri). Toxicol Appl Pharmacol 49:437–450

    PubMed  Google Scholar 

  • Glassen RC, Armstrong JE, Calder JA, Carter RWG, La Rock PA, Pilotte JO, Winchester JW (1977) Bayou Chico restoration study. Report to the Florida Department of Environmental Regulation, Florida State University Resources and Environmental Analysis Center, Tallahassee, Florida, pp 1–47

    Google Scholar 

  • Kezic N, Britvic S, Protic M, Simmons JE, Rijavec M, Zahn RK, Kurelec B (1983) Activity of benzo(a)pyrene monooxygenase in fish from Sava river, Yugoslavia: Correlation with pollution. Sci Total Environ 27:59–69

    PubMed  Google Scholar 

  • Kurelec B, Matijasevic Z, Rijavec M, Alacevic M, Britvic S, Muller WEG, Zahn RK (1979) Induction of benzo(a)pyrene monooxygenase in fish and the Salmonella test as a tool for detecting mutagenic/carcinogenic xenobiotics in the aquatic environment. Bull Environ Contam Toxicol 21:799–807

    PubMed  Google Scholar 

  • Lesko S, Caspary W, Lorentzen R, Ts'o POP (1975) Enzymic formation of 6-oxobenzo(a)pyrene radical in rat liver homogenates from carcinogenic benzo(a)pyrene. Biochem 14:3978–3984

    Google Scholar 

  • Malins DC, Krahn MM, Myers MS, Rhodes LD, Brown DW, Krone CA, McCain BB, Chan S-L (1985) Toxic chemicals in sediments from a creosote-polluted harbor: Relationships with hepatic neoplasms and other hepatic lesions in English sole (Parophrys vetulus). Carcinogenesis 6:1463–1469

    PubMed  Google Scholar 

  • Payne JF (1976) Field evaluation of benzopyrene hydroxylase induction as a monitor for marine petroleum pollution. Science 191:945–946

    PubMed  Google Scholar 

  • — (1984) Mixed-function oxygenases in biological monitoring programs: Review of potential usage in different phyla of aquatic animals. In: Ecotoxicological Testing for the Marine Environment, Persoone G, Jaspers E, Claus C (eds) State Univ Ghent and Inst Mar Scient Res, Bredene, Belgium, Vol. 1, 798 pp

    Google Scholar 

  • Payne JF, Bauld C, Dey AC, Kiceniuk JW, Williams U (1984) Selectivity of mixed-function oxygenase enzyme induction in flounder (Pseudopleuronectes americanus) collected at the site of the Baie Verte, Newfoundland oil spill. Comp Biochem Physiol 79:15–9

    Google Scholar 

  • Schoor WP (1984) Benzo(a)pyrene metabolism in marine fish and some analytical aspects of its metabolites. Natl Cancer Inst Monogr 65:391–396

    PubMed  Google Scholar 

  • Schoor WP, Srivastava M (1984) Position-specific induction of benzo(a)pyrene metabolism by 3-methylcholanthrene and phenobarbital in mullet (Mugil cephalus), a marine fish. Comp Biochem Physiol 78:391–6

    Google Scholar 

  • Stegeman JJ (1980) Mixed-function oxygenase studies in monitoring for effects of organic pollution. Rapp. P.-v. Reun. Cons. int. Explor. Mer 179:33–38

    Google Scholar 

  • Stegeman JJ, Klotz AV, Woodin BR, Pajor AM (1981) Induction of hepatic cytochrome P-450 in fish and the indication of environmental induction in scup (Stenotomus chrysops). Aquat Toxicol 1:197–212

    Google Scholar 

  • Stegeman JJ, Kloepper-Sams PJ, Farrington JW (1986) Monoxygenase induction and chlorobiphenyls in the deep-sea fishCoryphaenoides armatus. Science 231:1287–1289

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    PubMed  Google Scholar 

  • Varanasi U, Collier TK, Williams DE, Buhler DR (1986) Hepatic cytochrome P-450 isozymes and aryl hydrocarbon hydroxylase in the English sole (Parophrys vetulus). Biochem Pharmacol 35:2967–2971

    PubMed  Google Scholar 

  • Williams DE, Masters BSS, Lech JJ, Buhler DR (1986) Sex differences in cytochrome P-450 isozyme composition and activity in kidney microsomes of mature rainbow trout. Biochem Pharmacol 35:2017–2023.

    PubMed  Google Scholar 

  • Williams DE, Bender RC, Morrissey MT, Selivonchick DP, Buhler DR (1984) Cytochrome P-450 isozymes in salmonids determined with antibodies to purified forms of P-450 from rainbow trout. Mar Environ Res 14:13–21

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schoor, W.P., Williams, D.E. & Lech, J.J. Combined use of biochemical indicators to assess sublethal pollution effects on the Gulf killifish (Fundulus grandis). Arch. Environ. Contam. Toxicol. 17, 437–441 (1988). https://doi.org/10.1007/BF01055508

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01055508

Keywords

Navigation