Abstract
The immunomodulation response of mice to low levels of aldicarb in drinking water was investigated in four series of studies. The splenic plaque forming cell (PFC) response to red sheep cells were measured for treatment levels of 0.01 to 1,000 ppb (μg/kg). Based on their beginning and end body weights, the animal populations were uniform in all series of tests, but based on their net body weights and PFC counts they were highly nonuniform in the 30 and 60 day tests and uniform in the 90 and 180 day tests.
The mean PFC counts for animals in each treatment were calculated and compared with the mean PFC counts for animals in the controls in all four series of tests. This approach ignores the variability and nonuniformity in the animal population. The outcomes using this approach were stimulatory for the 30 and 60 day tests and inhibitory for the 90 and 180 day tests.
An alternative approach was developed based on the analysis of the distributions of the relative PFC counts of each animal in a treatment with each animal in a control, and specifically addresses the variability and nonuniformity in animal population as integral parts of the analysis. The distribution peaks were estimated by maximum likelihood and kernel/bootstrap procedures and were used to summarize the tests. The outcomes were consistently inhibitory, indicating immune suppression. The outcome of this approach converged to the outcome of the mean PFC approach for the 90 and 180 day tests where the animal populations were uniform.
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Conkling P, Cornwall DG, Sagone AL Jr (1985) Effect of diethyldithiocarbamate on Human Monocyte Function and Metabolism. Inflammation 9(2):149–161
Devroye L (1987) A Course in Density Estimation. Birkhauser, Boston
Fukanaga K (1972) Introduction to Statistical Pattern Recognition. Academic Press, New York
Mosteller F, Tukey JF (1977) Data Analysis and Regression. Addison-Wesley, Menlo Park, CA
Olson LJ, Erickson BJ, Hinsdill RD, Wyman JA, Porter WP, Binning LK, Bidgood RC, Nordheim EV (1987) Aldicarb Immunomodulation in Mice: An Inverse Dose-Response to Parts Per Billion Levels in Drinking Water. Archives Environ Contam Toxicol 16:433–439
Rigas DA, Eginitis-Rigas C, Head C (1979) Biphasic Toxicity of Diethyldithiocarbamate, a Metal Chelator to T Lymphocytes and Polymorphonuclear Granulocytes: Reversal by Zinc and Copper. Biochemical and Biophysical Research Communications 8(22):373–379
Silverman BW (1986) Density Estimation for Statistics and Data Analysis. Chapman and Hall, New York
Thomas PT, Ratajczack HV, Eisenberg WC, Furedi-Machacek M, Ketels KV, Barbera PW (1987) Evaluation of Host Resistance and Immunity in Mice Exposed to the Carbamate Pesticide Aldicarb. Fundamental Applied Toxicol 9:82–89
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Shirazi, M.A., Erickson, B.J., Hinsdill, R.D. et al. An analysis of risk from exposure to aldicarb using immune response of nonuniform populations of mice. Arch. Environ. Contam. Toxicol. 19, 447–456 (1990). https://doi.org/10.1007/BF01054991
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DOI: https://doi.org/10.1007/BF01054991