Abstract
Lake trout samples from the Great Lakes contain residues of a complex pattern of chlorinated bornanes similar to the insecticide toxaphene. These residues, although structurally similar to toxaphene, are composed of a different profile of compounds than the analytical standard. Chlorinated bornane residues were isolated from tissues of Lake Michigan and Siskiwit Lake (Isle Royale) lake trout using a variety of purification techniques. Toxicity studies were conducted to determine the toxicological properties of the isolated residues in comparison to both technical material and the procedural standard. Static 24-hr acute bioassays with mosquito larvae(Aedes egypti) demonstrated that the residues were as toxic as the toxaphene standard. Additionally, experiments performed on the picrotoxinin receptor of the GABA (γ-aminobutyric acid)-chloride ionophore complex of the central nervous system revealed that the residues were very potent at the [35S]-t-butylbicyclophosphorothionate binding site. Therefore, both non-specific and specific measures of toxicologic potency show that environmentally derived residues of toxaphene retain significant biologic activity. There is no trend for decreasing toxicity of toxaphene residues for the period of 1982–85. Also, the residues found in samples from Siskiwit Lake were as toxic as those found in Lake Michigan.
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References
Abalis IM, Eldefrawi ME, Eldefrawi AT (1985) High affinity stereospecific binding of cyclodiene insecticides and -γ-hexachlorocyclohexane to γ-aminobutyric acid receptors of rat brain. Pestic Biochem Physiol 23:
Clark JM, Matsumura F (1979) Metabolism of toxaphene by aquatic sediment and a camphor-degrading pseudomonad. Arch Environ Contam Toxicol 8:285–298
Cole LM, Lawrence LJ, Casida JE (1984) Similar properties of35S]t-butylbicyclophosphorothionate receptor and coupled components of the GABA-receptor ionophore complex in brains of human, cow, rat, chicken and fish. Life Sci 35:1755–1762
Farrell TJ (1985) Toxaphene-chlordane separation and toxaphene determination in Great Lakes fish. Abstract, Sixth Annual Meeting Society of Environmental Toxicology and Chemistry, St. Louis, MO, November 1985
Ghiasuddin SM, Matsumura F (1982) Inhibition of gamma-aminobutyric acid (GABA)-induced chloride uptake by gamma-BHC and heptachlor epoxide. Comp Biochem Physiol 73C:141–144
Gooch JW, Matsumura F (1985) Evaluation of the toxic components of toxaphene in Lake Michigan lake trout. J Agric Food Chem 33:844–848
Harder HW, Carter TV, Bidleman TF (1983) Acute effects of toxaphene and its sediment degraded products on estuarine fish. Can J Fish Aquat Sci 40:2119–2125
Holdrinet MVH (1979) Confirmation of mirex andcis- and trans-chlordane in the presence of other organochlorine insecticides and polychlorinated biphenyls. Bull Environ Contam Toxicol 21:46–52
Isensee AR, Jones GE, McCann JA, Pitcher FG (1979) Toxicity and fate of nine toxaphene fractions in an aquatic model ecosystem. J Agric Food Chem 27:1041–1046
Jansson B, Vaz R, Blomkvist G, Jensen S, Olsson M (1979) Chlorinated terpenes and chlordane components found in fish, guillemot and seal from Swedish waters. Chemosphere 4:181–190
Johnson WD, Lee GF, Spyridakis D (1966) Persistence of toxaphene in treated lakes. Air Water Pollut Int J 10:555–560
Klein AL, Link JD (1970) Elimination of interferences in the determination of toxaphene residues. J Assoc Office Anal Chem 53:524–529
Kramer W, Buchert H, Reuter U, Biscoito M, Maul DG, Le Grand G, Ballschmiter K (1984) Global baseline pollution studies IX: C6–C14 organochlorine compounds in surface-water and deep-sea fish from the eastern North Atlantic. Chemosphere 13:1255–1267
Lawrence LJ, Casida JE (1984) Interactions of lindane, toxaphene, and cyclodienes with brain-specific t-butylbicyclo-phosphorothionate receptor. Life Sci 35:171–178
Matsumura F, Ghiasuddin S (1983) Evidence for similarities between cyclodiene type insecticides and picrotoxinin in their action mechanisms. J Environ Sci Health B18:1–14
Matsumura F, Tanaka K (1984) Molecular basis of neuroexcitatory actions of cyclodiene-type insecticides. In: Narahashi T (ed) Cellular and Molecular Neurotoxicology, Raven Press, New York, pp 225–240
Musial CJ, Uthe JF (1983) Widespread occurrence of toxaphene in Canadian east coast marine fish. Int J Environ Anal Chem 14:117–126
Nelson JO, Matsumura F (1975) A simplified approach to studies of toxic toxaphene components. Bull Environ Contam Toxicol 13:464–470
Parr JF, Smith S (1976) Degradation of toxaphene in selected anaerobic soil environments. Soil Sci 121:52–57
Ramanjaneyulu R, Ticku MK (1984) Binding characteristics and interactions of depressant drugs with [35S]t-butybicyclo-phosphorothionate, a ligand that binds to the picrotoxinin site. J Neurochem 42:221–229
Ribick MA, Dubay GR, Petty JD, Stalling DL, Schmitt CJ (1982) Toxaphene residues in fish: Identification, quantification, and confirmation at part per billion levels. Environ Sci Technol 16:310–318
Ribick MA, Zajicek J (1983) Gas chromatographic and mass spectrometric identification of chlordane components in fish from Manoa Stream, Hawaii. Chemosphere 12:1229–1242
Rice CP, Evans MS (1984) Toxaphene in the Great Lakes. In: Nriagu JO, Simmons M (eds) Toxic contaminants in the Great Lakes. John Wiley and Sons, New York, Volume 14, Chapter 8
Saleh MA, Turner WV, Casida JE (1977) Polychlorobornane components of toxaphene: Structure toxicity relations and metabolic reductive dechlorination. Science 198:1256–1258
Squires RF, Casida JE, Richardson M, Saederup E (1983) [35S]t-butylbicyclophosphorothionate binds with high affinity to brain-specific sites coupled to γ-aminobutyric acid-A and ion recognition sites. Mol Pharmacol 23:326–336
Steel RGD, Torrie JH (1980) Principles and procedures of statistics: A biometrie approach. McGraw Hill, New York
Strachan WMJ (1985) Organic substances in the rainfall of Lake Superior 1983. Environ Toxicol Chem 4:677–683
Ticku MK, Olsen RW (1979) Cage convulsants inhibit picrotoxinin binding. Neuropharm 18:315–318
Turner WV, Engel JL, Casida JE (1977) Toxaphene components and related compounds: Preparation and toxicity of some hepta-, octa-, and nonachlorobornanes, hexa- and heptachlorobornenes and a hexachlorobornadiene. J Agric Food Chem 25:1394–1401
Underwood JC (1978) Charcoal column separation of chlordane and toxaphene. Bull Environ Contam Toxicol 20:445–446
Vaz R, Blomkvist G (1985) Traces of toxaphene components in Swedish breast milk analyzed by capillary GC using ECD electron impact and negative chemical ionization MS. Chemosphere 14:223–231
Wideqvist U, Jansson B, Reutergardh L, Sundstrom G (1984) The evaluation of an analytical method for polychlorinated terpenes (PCC) in biological samples using an internal standard. Chemosphere 13:367–379
Zell M, Ballschmiter K (1980) Baseline studies of global pollution II. Global occurrence of hexachlorobenzene (HCB) and polychloro-camphenes (toxaphene) (PCC) in biological samples. Fres Z Anal Chem 300:387–402
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Gooch, J.W., Matsumura, F. Toxicity of chlorinated bornane (toxaphene) residues isolated from Great Lakes Lake trout (salvelinus namaycush). Arch. Environ. Contam. Toxicol. 16, 349–355 (1987). https://doi.org/10.1007/BF01054953
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DOI: https://doi.org/10.1007/BF01054953