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Growth inhibition of human glioma cells modulated by retrovirus gene transfection with antisense IL-8

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Abstract

The human glioma cell line, NP-1, expresses IL-8 mRNA and constitutively secretes IL-8 protein. Administration of recombinant IL-8 increased the proliferation of NP-1 cells in a dose-dependent manner. In the presence of anti-IL-8 antiserum, the IL-8 mediated proliferation of NP-1 cell growth was inhibited. Further, NP-1 cell growth was inhibited by transfection of retroviral constructs encoding antisense IL-8 bothin vitro andin vivo models. These results suggest that antisense IL-8 gene therapy could be beneficial in gliomas where autocrine stimulation by IL-8 is implicated.

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Authors and Affiliations

  1. Department of Neurosurgery, Brain Research Institute, Niigata, Japan

    Ryuya Yamanaka, Ryuichi Tanaka, Seiichi Yoshida & Takafumi Saitoh

  2. Department of Obstetrics and Gynecology, Niigata University, Niigata, Japan

    Kazuyuki Fujita

Authors
  1. Ryuya Yamanaka
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  2. Ryuichi Tanaka
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  3. Seiichi Yoshida
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  4. Takafumi Saitoh
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  5. Kazuyuki Fujita
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Yamanaka, R., Tanaka, R., Yoshida, S. et al. Growth inhibition of human glioma cells modulated by retrovirus gene transfection with antisense IL-8. J Neuro-Oncol 25, 59–65 (1995). https://doi.org/10.1007/BF01054723

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