Abstract
To find out glycoproteins involved in the mating reaction ofChlamydomonas reinhardtii, the effect of tunicamycin (TM), a potent inhibitor of glycosylation of proteins, was studied. TM, when present during gametogenesis, blocked the acquisition of agglutinability ofmt + cells. TM also inhibited the recovery of agglutinability ofmt + gamete after trypsin treatment. On the contrary, TM blocked neither the acquisition of agglutination during gametogenesis ofmt - cells nor the recovery of their agglutinability after trypsinization. It was found, however, that the TM-treatedmt - gametes can agglutinate but do not fuse with non treatedmt + gametes at all. When gametes of gam-1mt -, a conditional mutant strain for cell fusion, were induced at non permissive temperature of 35°C and then transferred to 25°C, the ability of cell fusion was acquired after about 5 h incubation. Presence of TM completely blocked this acquisition. Based on these evidence, we conclude that at least two TM-sensitive glycoproteins are included in the mating reaction. The first component is located on the flagellar surface ofmt + gamete and responsible for agglutination withmt - flagella. The second component occurs on the surface ofmt - gamete and plays a role in the fusion withmt + gamete.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- CHI:
-
cycloheximide
- mt :
-
mating type
- TM:
-
tunicamycin
References
Bergman K, Goodenough UW, Goodenough DA, Jawitz J, Martin H (1975) Gametic differentiation inChlamydomonas reinhardtii. II. Flagellar membranes and the agglutination reaction. J Cell Biol 67:606–622
Claes H (1971) Autolyse der Zellwand bei den Gameten vonChlamydomonas reinhardii. Arch Mikrobiol 78:180–188
Forest CL, Goodenough DA, Goodenough UW (1978) Flagellar membrane agglutination and sexual signaling in the conditional gam-1 mutant ofChlamydomonas. J Cell Biol 79:74–84
Forest CL, Togasaki RK (1975) Selection for conditional gametogenesis inChlamydomonas reinhardii. Proc Natl Acad Sci USA 72:3652–3655
Goodenough UW (1977) Mating interactions inChlamydomonas. In: JL Reissig (ed) Microbial interactions, receptors and recognition, series B Vol 3. Chapman and Hall, London, pp 324–350
Goodenough UW (1980) Sexual microbiology: Mating reactions ofChlamydomonas reinhardii, Tetrahymena thermophila andSaccharomyces cerevisiae. In: GW Gooday, D Lloyd, APJ Trinci (eds) The eukaryotic microbial cell. Cambridge Univ Press, Cambridge, pp 301–328
Goodenough UW, Weiss RL (1975) Gametic differentiation inChlamydomonas reinhardtii. III. Cell wall lysis and microfilament-associated mating structure activation in wild-type and mutant strains. J Cell Biol 67:623–637
Kobata A (1979) Structures and functions of the sugar chains of cell surface glycoproteins. Cell Struct Funct 4:169–181
Mahoney WC, Duksin D (1979) Biological activities of the two major components of tunicamycin. J Biol Chem 254:6572–6576
Matsuda Y, Kikuchi T, Ishida MR (1971) Studies on chloroplast development inChlamydomonas reinhardtii. I. Effect of brief illumination on chlorophyll synthesis. Plant Cell Physiol 12:127–135
Matsuda Y, Sakamoto K, Mizuochi T, Kobata A, Tamura G, Tsubo Y (1981) Mating type specific inhibition of gametic differentiation ofChlamydomonas reinhardtii by tunicamycin. Plant Cell Physiol 22:1607–1611
Matsuda Y, Tamaki S, Tsubo Y (1978) Mating type specific induction of cell wall lytic factor by agglutination of gametes inChlamydomonas reinhardtii. Plant Cell Physiol 19:1253–1261
Ray DA, Solter KM, Gibor A (1978) Surface glycoproteins in mating ofChlamydomonas reinhardii. J Cell Biol 79:164a
Schlösser UG (1976) Entwicklungsstadien- und sippenspezifische Zellwand-Autolysine bei der Freisetzung von Fortpflanzungszellen in der GattungChlamydomonas. Ber Deutsch Bot Ges 89:1–56
Snell WJ (1976) Mating inChlamydomonas: A system for the study of specific cell adhesion. I. Ultrastructural and electrophoretic analyses of flagellar surface components involved in adhesion. J Cell Biol 68:48–69
Solter KM, Gibor A (1978) Removal and recovery of mating receptors on flagella ofChlamydomonas reinhardii. Exp Cell Res 115:175–181
Struck DK, Lennarz WJ (1980) The function of saccharide-lipids in synthesis of glycoproteins. In: WJ Lennarz (ed) The biochemistry of glycoproteins and proteoglycans. Plenum press, New York London, pp 35–83
Tamaki S, Matsuda Y, Tsubo Y (1981) The isolation and properties of the lytic enzyme of the cell wall released by mating gametes ofChlamydomonas reinhardtii. Plant Cell Physiol 22:127–133
Wiese L (1965) On sexual agglutination and mating-type substances (gamones) in isogamous heterothallicChlamydomonas. I. Evidence of the identity of the gamones with the surface components responsible for sexual flagellar contact. J Phycol 1:46–54
Wiese L (1974) Nature of sex specific glycoprotein agglutinins inChlamydomonas. Ann NY Acad Sci 234:383–395
Wiese L, Hayward PC (1972) On sexual agglutination and mating-type substances in isogamous dioeciousChlamydomonas. III. The sensitivity of sex cell contact to various enzymes. Amer J Bot 59:530–536
Wiese L, Wiese W (1978) Sex cell contact inChlamydomonas, a model for cell recognition. In: ASG Curtis (ed) Cell-cell recognition. Cambridge Univ Press, Cambridge, pp 83–104
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Matsuda, Y., Sakamoto, K., Kiuchi, N. et al. Two tunicamycin-sensitive components involved in agglutination and fusion ofChlamydomonas reinhardtii gametes. Arch. Microbiol. 131, 87–90 (1982). https://doi.org/10.1007/BF01053986
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01053986