Abstract
Sialic acids and the majorO-glycosidic oligosaccharide of glycophorin MK from monkey (Japanese monkey,Macaca fuscata) erythrocyte membranes were characterized.N-Glycolylneuraminic acid (neu5Gc) was found as the major sialic acid, which was confirmed by gas-liquid chromatography-mass spectrometry as the trimethylsilyl methyl ester. ThreeO-glycosidic oligosaccharide units were obtained from a tryptic glycopeptide that contained all of the carbohydrate units in glycophorin MK by mild alkaline borohydride/borotritide treatment. Carbohydrate analyses of the oligosaccharides revealed that they were composed of Neu5Gc, galactose andN-acetylgalactosaminitol in the molar ratios of 1∶1∶1 (trisaccharide), 2∶1∶1 (tetrasaccharide) and 1∶1∶1 (pentasaccharide). The content of oligosaccharide units was estimated to be 1∶12∶5 for penta-, tetra- and trisaccharide, respectively, based on the yields, the molecular weight, and the number of oligosaccharide attachment sites in the amino-acid sequence. The tetrasaccharide was the major oligosaccharide and its structure was proposed to be Neu5Gcα2-3Galβ1-3[Neu5Gcα2-6]GalNAcol.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Robinson J, Sieff C, Delia D, Edwards PWA, Greaves M (1981) Nature 289:119–21.
Aminoff D, von der Bruegge WF, Bell WC, Sarpolis K, Williams R (1977) Proc Natl Acad Sci USA 74:1521–24.
Paulson JC (1985) in The Receptors, part II, ed. Conn M, Academic Press, New York, p 131–219.
Tomita M, Marchesi VT (1975) Proc Natl Acad Sci USA 72:2964–68.
Murayama JI, Tomita M, Hamada A (1982) J Membr Biol 64:205–15.
Honma K, Tomita M, Hamada A (1980) J Biochem (Tokyo) 88:1679–91.
Murayama JI, Yamashita T, Tomita M; Hamada A (1982) Biochim Biophys Acta 742:477–83.
Fukuda K, Tomita M, Hamada A (1980) J Biochem (Tokyo) 87:687–93.
Kawashima I, Fukuda K, Tomita A, Hamada A (1982) J Biochem (Tokyo) 91:865–72.
Fukuda K, Kawashima I, Tomita M, Hamada A (1982) Biochim Biophys Acta 717:278–88.
Yamashita T, Murayama JI, Utsumi H, Hamada A (1985) Biochim Biophys Acta 839:26–31.
Krotkiewski H (1988) Glycoconjugate J 5:35–48.
Murayama JI, Utsumi H, Hamada A (1989) Biochim Biophys Acta, in press.
Blumenfeld OO, Adamany AM, Puglia KV, Socha WW (1983) Biochem Genet 21:333–48.
Dodge JT, Mitchell C, Hanahan DJ (1963) Arch Biochem Biophys 100:119–30.
Marchesi VT, Andrews EP (1971) Science 174:1247–48.
Reuter G, Vliegenthart JFG, Wember M, Schauer R, Howard RJ (1980) Biochem Biophys Res Commun 94:567–72.
Casals-Stenzel J, Buscher HP, Schauer R (1975) Anal Biochem 65:507–24.
Kamerling JP, Vliegenthart JFG, Versluis C, Schauer R (1975) Carbohydr Res 41:7–17.
Stellner K, Saito H, Hakomori S (1973) Arch Biochem Biophys 155:464–72.
Endo Y, Yamashita K, Han YN, Iwanaga S, Kobata A (1977) J Biochem (Tokyo) 82:545–50.
Reinhold VN (1972) Methods Enzymol 25:244–49.
Uemura K, Yuzawa M, Taketomi T (1978) J Biochem (Tokyo) 83:463–71.
Kamerling JP, Vliegenthart JFG (1974) Carbohydr Res 33:297–306.
Inoue S, Matsumura G (1979) Carbohydr Res 74:361–68.
Buscher HP, Casals-Stenzel J, Schauer R (1974) Eur J Biochem 50:71–82.
Corfield AP, Schauer R (1982) Sialic Acids, Cell Biology Monographs, Vol. 10, ed. Schauer R, Springer Verlag, p 5–50.
Yasue S, Handa S, Miyagawa S, Inoue J, Hasegawa A, Yamakawa T (1978) J Biochem (Tokyo) 83:1101–7.
Varki A, Kornfeld S (1980) J Exp Med 152:532–44.
Thomas DB, Winzler RJ (1969) J Biol Chem 244:5943–46.
Fukuda M, Lauffenburger M, Sasaki H, Rogers ME, Dell A (1987) J Biol Chem 262:11952–57.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Murayama, JI., Manabe, H., Fukuda, K. et al. Structure of the majorO-glycosidic oligosaccharide of monkey erythrocyte glycophorin. Glycoconjugate J 6, 499–510 (1989). https://doi.org/10.1007/BF01053773
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01053773