Abstract
Participation of the brain-specific non-histone chromatin proteins Np-3.5 in the reproduction of an averse habit to certain kind of food was studied in experiments onHelix lucorum. Antibodies to these proteins were found to completely suppress the behavioral reactions and responses of the defensive behavior command neurons evoked by a presentation of a certain conditioning stimulus: a carrot. The action of the antibodies was specific, as they did not change the snail's reactions to presentation of another conditioning stimulus: an apple. The effect developed 1.5 h after the administration of antibodies to Np-3.5, and remained for 80–110 min, with subsequent complete restoration of the conditioned reflex. The antibodies to Np-3.5 did not affect the nutritive behavior of untrained snails. The antibodies to Np-3.5 were found to label cytoplasm in the command neurons, whereas in the snails trained to reject carrot the label appeared in the nuclei of the cells. It has been suggested that the Np-3.5 proteins selectively participate in the molecular-genetic processes responsible for neurophysiological mechanisms of an information draw from the long-term memory.
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Kozyrev, S.A., Nikitin, V.P., Goncharuk, V.D. et al. Selective involvement of non-histone chromatin proteins in the reproduction of averse reaction to food in snail. Neurophysiology 27, 132–141 (1995). https://doi.org/10.1007/BF01053167
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DOI: https://doi.org/10.1007/BF01053167