Abstract
Using the voltage clamp technique under conditions of intracellular perfusion, we investigated changes in high-threshold voltage-dependent calcium current (ICa) in the surface membrane of PC 12 cells caused by intracellular administration of the aromatic amino acids L-tyrosine and L-phenylalanine. Administration of L-tyrosine (20 mmole/1) prevented decrease in ICa caused by perfusion of the cell with an artificial saline solution and had a transient restorative effect upon the cell. Administration of L-phenylalanine (20 mM) quickened the decrease in amplitude of ICa observed in the control. These effect of aromatic amino acids are maintained when ATP (2 mM) in the intracellular solution is replaced by an equivalent quantity of ADP. The tyrosine hydroxylase blocker α-methyl D,L-tyrosine (20 MM) had an effect upon ICa analogous to that of L-tyrosine.
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A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 23, No. 1, pp. 105–111, January–February, 1991.
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Martynyuk, A.É., Pogorelaya, N.K. Effect of intracellular administration of L-tyrosine and L-phenylalanine upon voltage-dependent calcium current in PC 12 pheochromocytoma cells. Neurophysiology 23, 89–95 (1991). https://doi.org/10.1007/BF01052295
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DOI: https://doi.org/10.1007/BF01052295