Abstract
Dependence of the blocking effect of dopamine on the calcium current on guanosine triphosphate (GTP) was investigated on dialyzed neurons ofLymnaea stagnalis. Against the background of the effect of the nonhydrolyzable GTP analog guanosine-5′-0-3-thiotriphosphate (GTPγS) (10−4 M) marked residual blocking of the calcium current was observed during rinsing out of dopamine, and this was potentiated in the case of repeated application of the hormone. It is suggested that the receptor-mediated effect of GTPγS on the calcium current is effected through activation of phospholipase C.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
A. R. Akopyan, V. I. Il'in, and N. K. Chemeris, "Modulation by dopamine of the calcium component of the action potential in the molluscan neuron soma," Biofizika,29, No. 2, 284 (1984).
P. G. Kostyuk, Calcium and Cellular Excitability [in Russian], Nauka, Moscow (1986).
V. G. Safronova and N. K. Chemeris, "Modulation of the calcium current by dopamine: role of intracellular calcium," Dokl. Akad. Nauk SSSR,294, No. 6, 1513 (1987).
N. K. Chemeris, V. G. Safronova, and A. A. Selishcheva, "Role of phosphoinositide metabolism in blocking of the calcium current by dopamine," Dokl. Akad. Nauk SSSR,296, No. 4, 1008 (1987).
N. K. Chemeris, V. G. Safronova, A. A. Selishcheva, and V. I. Il'in, "Modulation of the voltage-dependent calcium current in neurons ofLymnaea stagnalis by dopamine and and products of phosphatidylinositide metabolism," Intracellular Signalization [in Russian], Nauka, Moscow (1988), pp. 32–41.
J. A. Allende, "GTP-mediated macromolecular interaction: the common features of different systems," FASEB J.,2, No. 8, 2366 (1988).
A. M. Brown and L. Birnbaumer, "Direct G protein gating of ion channels," Am. J. Physiol.,254, No. 3, H401 (1988).
K. Dunlap, G. G. Holtz, and S. G. Rain, "G proteins as regulators of ion channel function," Tr. Neurosci.,10, No. 6, 241 (1987).
A. G. Gilman, "G Protein: transducers of receptor-generated signals," Ann. Rev. Biochem.,56, 615 (1987).
M. A. Kostenko, V. I. Geletyuk, and B. N. Veprintsev, "Completely isolated neurons in the molluscLymnaea stagnalis. A new objective for nerve cell biology investigation," Comp. Biochem. Physiol.,49, 89 (1974).
P. G. Kostyuk and O. A. Krishtal, "Effects of calcium and calcium-chelating agents on the inward and outward current in the membrane of mollusc neurones," J. Physiol. (London),270, No. 3, 569 (1977).
D. L. Lewis, F. F. Weight, and A. Luini, "A guanine nucleotide-binding protein mediates the inhibition of voltage-dependent calcium current by somatostatin in a pituitary cell line," Proc. Natl. Acad. Sci., USA,83, 9035 (1986).
H. Reuter, "Calcium channel modulation by neurotransmitters, enzymes and drugs," Nature,301, 569 (1983).
R. H. Scott and A. C. Dolphin, "Regulation of calcium currents by a GTP analogue: potentiation of (-)-baclofen-mediated inhibition," Neurosci. Lett.,69, 59 (1986).
C. D. Smith, C. Cox, and R. Snyderman, "Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N-protein," Science,232, 97 (1986).
Additional information
Institute of Biophysics, Academy of Sciences of the USSR, Pushchino, Moscow. Translated from Neirofiziologiya, Vol. 23, No. 1, pp. 43–48, January–February, 1991.
Rights and permissions
About this article
Cite this article
Safronova, V.G., Chemeris, N.K. Action of a nonhydrolyzable guanosine triphosphate analog on modulation of calcium current by dopamine in Lymnaea stagnalis neuron. Neurophysiology 23, 36–39 (1991). https://doi.org/10.1007/BF01052287
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01052287