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Different susceptibilities of complex-, hybrid- and high-mannose-type α1- inhibitor and α1-acid glycoprotein to endo-β-N-acetylglucosaminidase F and peptide:N-glycosidase F

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Abstract

Endo-β-N-acetylglucosaminidase F (endo F, EC 3.2.1.96) and peptide:N-glycosidase F (PNGase F, EC 3.2.2.18) fromFlavobacterium meningosepticum were used for the deglycosylation of α1-proteinase inhibitor and α1-acid glycoprotein carrying oligosaccharide side chains of the complex-, high-mannose- and hybrid-type. High-mannose-and hybrid-type glycoproteins were obtained by the incubation of rat hepatocyte primary cultures with 1-deoxymannojirimycin or swainsonine, respectively. It was found that endo F cleaves hybrid- and high-mannose-type α1-proteinase inhibitor and α1-acid glycoprotein at pH 4.5 as well as at pH 8.5 in the presence or absence of 1% octyl-β-d-glucopyranoside. Complex-type α1-proteinase inhibitor or α1-acid glycoprotein were not cleaved by endo F even in the presence of octyl-β-d-glucopyranoside.

PNGase F was found to cleave complex-, hybrid- and high-mannose-type oligosaccharide side chains of α1-proteinase inhibitor and α1-acid glycoprotein at pH 4.5 and pH 8.5 in the presence of 0.75% octyl-β-d-glucopyranoside. The deglycosylation of both protein substrates was very poor without detergents.

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Abbreviations

Endo F:

endo-β-N-acetylglucosaminidase F (EC 3.2.1.96)

PNGase F:

peptide:N-glycosidase F (EC 3.2.2.18)

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Authors and Affiliations

  1. Biochemisches Institut, Universität Freiburg, Hermann-Herder-Str. 7, D-7800, Freiburg, W. Germany

    Klaus Steube, Thuy-anh Tran-Thi, Karl Decker & Peter C Heinrich

  2. Medizinische Universitätsklinik, Hugstetter Str. 55, D-7800, Freiburg, W. Germany

    Volker Gross

  3. Boehringer Mannheim, Biochemica Werk Tutzing, Bahnhofstr. 9-15, D-8132, Tutzing, W. Germany

    Wolfgang Hösel

Authors
  1. Klaus Steube
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  2. Volker Gross
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  3. Wolfgang Hösel
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  4. Thuy-anh Tran-Thi
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  5. Karl Decker
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  6. Peter C Heinrich
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Dedicated to Prof. Dr. Wolfgang Gerok on the occasion of his 60th birthday

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Steube, K., Gross, V., Hösel, W. et al. Different susceptibilities of complex-, hybrid- and high-mannose-type α1- inhibitor and α1-acid glycoprotein to endo-β-N-acetylglucosaminidase F and peptide:N-glycosidase F. Glycoconjugate J 3, 247–254 (1986). https://doi.org/10.1007/BF01051775

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  • DOI: https://doi.org/10.1007/BF01051775

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