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Isolation and characterisation of three fucosyloligosaccharide-1-phosphates from normal human urine

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Abstract

Three phosphate-containing fucosyloligosaccharides were isolated from normal human urine using charcoal adsorption, gel-filtration, ion-exchange chromatography and paper chromatography. Chemical investigations and 400 MHz1H-NMR spectroscopy analyses led to the following structures:

Their oligosaccharide chains are identical with, or similar to the fucosyloligosaccharides and urinary compounds synthesized by the stepwise transfer ofN-acetylglucosamine, galactose, fucose andN-acetylgalactosamine to free galactose or glucose residues. A transfer reaction of monosaccharides to free hexose-1-phosphates or glycosylnucleotides is proposed for explaining the origin of these sugar-phosphates.

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Authors and Affiliations

  1. Laboratoire de Chimie Biologique (Unit Mixte du CNRS no. 111), Université des Sciences et Techniques de Lille Flandres-Artois, 59655, Villeneuve d'Ascq Cedex, France

    Gérard Strecker, Jean-Michel Wieruszeski, Jean-Claude Michalski & Jean Montreuil

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  1. Gérard Strecker
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  2. Jean-Michel Wieruszeski
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  3. Jean-Claude Michalski
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  4. Jean Montreuil
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Strecker, G., Wieruszeski, JM., Michalski, JC. et al. Isolation and characterisation of three fucosyloligosaccharide-1-phosphates from normal human urine. Glycoconjugate J 7, 235–245 (1990). https://doi.org/10.1007/BF01050607

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  • DOI: https://doi.org/10.1007/BF01050607

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