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Carbohydrate exposure of human promyelocytic HL60 cells and histiocytic U937 cells during phagocytic differentiation assessed with fluoresceinated lectins

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Abstract

The display of carbohydrate structures was measured in promyelocytic HL60 cells and in histiocytic U937 cells induced to differentiate to phagocytic cellsin vitro during three to seven days of cultivation in the presence of dimethylsulfoxide (DMSO). It was assessed by micro-or spectrofluorometric quantification of the binding of fluorescent lectins. Changes in the cell size and the association and uptake of IgG-or complementopsonized yeast cells (Saccharomyces cerevisiae) were used as signs of phagocyte differentiation.

The binding of wheat germ agglutinin (WGA), concanavalin A (Con A),Ricinus communis agglutinin-I (RCA-I) andUlex europaeus agglutinin-I (UEA-I) varied due to the presence of DMSO during cultivation, and without DMSO also on the number of days in culture and the type of cell.

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Abbreviations

DMSO:

dimethylsulfoxide

PMA:

phorbol 12-myristate 13-acetate

KRG:

Krebs-Ringer phosphate buffer with glucose

WGA:

wheat germ agglutinin

Con A:

concanavalin A

RCA-I:

Ricinus communis agglutinin-I

UEA-I:

Ulex europaeus agglutinin-I

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Authors and Affiliations

  1. Department of Medical Microbiology, University of Linköping, S-581, Linköping, Sweden

    Karl-Erik Magnusson & Olle Stendahl

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  1. Karl-Erik Magnusson
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  2. Olle Stendahl
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Magnusson, KE., Stendahl, O. Carbohydrate exposure of human promyelocytic HL60 cells and histiocytic U937 cells during phagocytic differentiation assessed with fluoresceinated lectins. Glycoconjugate J 4, 203–210 (1987). https://doi.org/10.1007/BF01049457

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  • DOI: https://doi.org/10.1007/BF01049457

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