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Cell surface glycosaminoglycans of a tumor cell line and its DNA transfected variant differing in their lung colonizing potential

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Abstract

A highly lung-colonizing cell line RMS/82 was obtained by DNA transfection from a low lung-colonizing line RMS/8, a clone of a rat rhabdomyosarcoma cell line. The cells were metabolically labeled with3H-glucosamine and35S-sulfate. The newly synthesized pericellular glycosaminoglycans and the ability of the cells from the two lines to degrade extracellular matrix components were studied comparatively. The following conclusions were obtained: 1) Thein vitro proliferation rate is not a determinant in the modulation of the colonizing potential of these cells; 2) The strongly colonizing RMS/82 cells release more radioactivity from the radiolabeled extracellular matrix than their weakly colonizing counterparts; 3) The cells with a high colonizing potential incorporated less radioactivity into the cell surface glycosaminoglycans, and exhibited a lower heparan sulfate to chondroitin sulfate ratio than the weakly colonizing RMS/8 line.

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Abbreviations

RMS:

rhabdomyosarcoma

ECM:

extracellular matrix

GAGs:

glycosaminoglycans

DMEM:

Dulbecco's modified essential medium

FCS:

fetal calf serum

PBS:

phosphate buffered saline

CPC:

cetylpyridinium chloride

References

  1. Alitalo K, Vaheri A (1982) Adv Cancer Res 37:111–58.

    PubMed  Google Scholar 

  2. Smets LA, van Beek WP (1984) Biochim Biophys Acta 738:237–49.

    PubMed  Google Scholar 

  3. Yamada KM, Pouyssegur J (1978) Biochimie 60:1221–33.

    PubMed  Google Scholar 

  4. Iozzo RV (1985) J Biol Chem 260:7464–73.

    PubMed  Google Scholar 

  5. Pauli BU, Schwartz DE, Thonar EJ, Kuettner KE (1983) Cancer Metastasis Rev 2:12429–52.

    Google Scholar 

  6. Redini F, Moczar E, Poupon MF (1986) Biochim Biophys Acta 883:98–105.

    PubMed  Google Scholar 

  7. Moczar E, Becker M, Poupon MF (1985) Clin Exp Metastasis 3:235–45.

    PubMed  Google Scholar 

  8. Poupon MF, Becker M, Pauwels C, Moczar E, Korach S (1984) Bull Cancer (Paris) 71:453–63.

    Google Scholar 

  9. Becker M, Moczar M, Poupon MF, Moczar EJ (1986) J Natl Cancer Inst 77:417–24.

    PubMed  Google Scholar 

  10. Liotta LA, Kleinerman J, Catanzaro P, Rynbrandt D (1977) J Natl Cancer Inst 58:1427–31.

    PubMed  Google Scholar 

  11. Kramer RH, Vogel KG, Nicolson GL (1982) J Biol Chem 257:2678–86.

    PubMed  Google Scholar 

  12. Nicolson GL (1982) J Histochem Cytochem 30:214–20.

    PubMed  Google Scholar 

  13. Sweeney FL, Pot-Deprun J, Poupon MF, Chouroulinkov I (1980) Cancer Res 42:3776–800.

    Google Scholar 

  14. Becker M, Moczar E, Korach S, Lascaux V, Poupon MF (1985) in Treatment of Metastasis: Problems and Prospects, eds. Hellmann K, Eccles SA, Taylor and Francis, London, p 183–86.

    Google Scholar 

  15. Verrelle P, Lascaux V, Poupon MF, Hillova J (1987) Anticancer Res, in press.

  16. Graham FL, van der Eb Al (1973) Virology 52:456–67.

    PubMed  Google Scholar 

  17. Wasteson Å, Uthne K, Westermann B (1976) Biochem J 136:1069–74.

    Google Scholar 

  18. Oohira A, Wight IN, McPherson S, Bornstein P (1982) J Cell Biol 92:357–67.

    PubMed  Google Scholar 

  19. Moczar E, Laurent M, Courtois Y (1981) Biochim Biophys Acta 675:132–39.

    PubMed  Google Scholar 

  20. Shively SE, Conrad HE (1976) Biochemistry 15:3932–42.

    PubMed  Google Scholar 

  21. Gamse G, Fromme HG, Kresse H (1978) Biochim Biophys Acta 177:152–54.

    Google Scholar 

  22. Saito H, Yamagata T, Suzuki S (1969) J Biol Chem 243:1536–42.

    Google Scholar 

  23. Ohya T, Kaneko Y (1970) Biochim Biophys Acta 198:607–9.

    PubMed  Google Scholar 

  24. Moczar E, Tassin J, Courtois Y (1983) Exp Cell Res 149:95–104.

    PubMed  Google Scholar 

  25. Kjellén L, Oldberg Å, Höök M (1980) J Biol Chem 155:10407–13.

    Google Scholar 

  26. Kjellén L, Petterson I, Höök M (1981) Proc Natl Acad Sci USA 78:5371–75.

    PubMed  Google Scholar 

  27. Piepkorn M, Carney H, Linker A (1985) Lab Invest 53:187–93.

    PubMed  Google Scholar 

  28. Matuoka K, Mitsui Y, Murota SI (1985) Cell Biol Int Rep 9:577–86.

    PubMed  Google Scholar 

  29. Underhill CB, Keller JM (1976) J Cell Physiol 89:53–64.

    PubMed  Google Scholar 

  30. Winkelhake JL, Nicolson GL (1976) J Natl Cancer Inst 56:285–91.

    PubMed  Google Scholar 

  31. Fidler IJ (1975) Cancer Res 35:218–24.

    PubMed  Google Scholar 

  32. Nicolson GL (1978) Biosci Rep 28:441–47.

    Google Scholar 

  33. Nicolson GL, Winkelhake JL, Nussey AC (1976) in Fundamental Aspects of Metastasis, ed. Weiss L, North-Holland, Amsterdam, p 291–303.

    Google Scholar 

  34. Halpern B, Pejsachowicz B, Febvre HL, Barski G (1966) Nature 209:157–59.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biochemie du Tissu Conjonctif, UA CNRS 1174, Université Paris XII, Faculté de Médecine, 8 rue du Général Sarrail, F-94010, Créteil, France

    F Redini & E Moczar

  2. ER 148 du CNRS, I.C.I.G., 14-16 Avenue Paul-Vaillant-Couturier, 94804, Villejuif Cedex, France

    P Verrelle & J Hillova

  3. ER 278 du CNRS, I.R.S.C., 7 rue Guy Mocquet, B.P. 8, 94802, Villejuif Cedex, France

    P Verrelle & M F Poupon

Authors
  1. F Redini
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  2. P Verrelle
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  3. J Hillova
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  4. M F Poupon
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  5. E Moczar
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Redini, F., Verrelle, P., Hillova, J. et al. Cell surface glycosaminoglycans of a tumor cell line and its DNA transfected variant differing in their lung colonizing potential. Glycoconjugate J 4, 191–201 (1987). https://doi.org/10.1007/BF01049456

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  • DOI: https://doi.org/10.1007/BF01049456

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