Summary
Several mutants ofStreptomyces aureofaciens strain were used for protoplast regeneration and plasmid transformation. All tested mutants (excepting R 8/26) were transformable by number of plasmids and shuttle vectors. The transformation of the CTC production strains by plasmid containing cloned CTC resistance gene resulted in 1,1–4 times higher antibiotic production. From the restriction analysis of plasmid, phage and chromosomal DNAs it was estimated, that all tested mutants normally contain the modification system analogous toNae I (Roberts, 1987). Mutant R 8/26 expresses not only complete restriction-modification system mentioned above but also potential second system restricting several actinophages.
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Godány, A., Nazarov, V., Oktavcová, B. et al. Streptomyces aureofaciens strains as hosts for cloning of genes affecting antibiotic production. Biotechnol Lett 13, 471–476 (1991). https://doi.org/10.1007/BF01049202
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DOI: https://doi.org/10.1007/BF01049202