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Developmental changes of blood group A-active glycosphingolipids with type 1 and type 2 chains in rat small intestine

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Abstract

Blood group A-active glycosphingolipids of the small intestine, A-6 and A-12, which have been characterized previously in the adult rat [Breimer ME, Hansson GC, Karlsson K-A, Leffler H (1982) J Biol Chem 257:906–12], were found to appear during postnatal development, using immunostaining on thin layer chromatograms with two monoclonal anti-A antibodies, A005 and A581. In this system, A005 was found to be specific for the A determinant based on the type 2 chain, while A581 reacted mainly with the A determinant based on the type 1 chain and only weakly with the A determinant based on the type 2 chain. A-6 Type 1 was detected first at 18 days after birth. Its concentration increased markedly during the fourth week. A-6 Type 2 was detected, at a very low level, in neonates. Its concentration increased between days 15 and 20 and then decreased almost to the neonate level by 28 days. Dodecaglycosylceramide A-12 followed the same pattern of reactivity as A-6 type 1 with A581, and remained strongly reactive with A005 after 20 days. Linear A-6 and branched A-12 appeared simultaneously. Antibodies directed against blood group H determinants based on the type 1 or type 2 chains did not detect any H structure which might have appeared as a precursor of either A-6 or A-12 at the early stages of postnatal development.

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Abbreviations

A-6, A-12, H-5, H-10 etc:

the glycolipids are abbreviated by giving blood group activity, and number of sugars (see also Fig. 1)

GM3 :

GM3-ganglioside, H3NeuAc-LcCer

PBS:

phosphate-buffered saline

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Authors and Affiliations

  1. INSERM U76, Centre National de Transfusion Sanguine, 6 rue Alexandre-Cabanel, 75739, Paris Cedex 15, France

    Daniele Bouhours & Jean-Francois Bouhours

  2. Department of Medical Biochemistry, University of Göteborg, Sweden

    Göran Larson & Gunnar C Hansson

  3. Department of Clinical Chemistry, University Hospital, Lund, Sweden

    Arne Lundblad

Authors
  1. Daniele Bouhours
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  2. Göran Larson
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  3. Jean-Francois Bouhours
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  4. Arne Lundblad
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  5. Gunnar C Hansson
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Bouhours, D., Larson, G., Bouhours, JF. et al. Developmental changes of blood group A-active glycosphingolipids with type 1 and type 2 chains in rat small intestine. Glycoconjugate J 4, 59–71 (1987). https://doi.org/10.1007/BF01048445

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