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The substrate specificity of the enzyme amyloglucosidase towards a pentadeoxy derivative of maltose

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Abstract

2,6-Dideoxy-4-O-(2,3-dideoxy-α-d-erythro-hexopyranosyl)-1,5-anhydro-d-arabino-hexitol (2) has been synthesized and characterized by1H-and13C-NMR spectroscopy. The compound is a substrate for the enzyme amyloglucosidase, AMG (EC.3.2.1.3) and the reaction kinetics have been determined using1H-NMR spectroscopy. Compound 2 is hydrolysed by AMG with a half time of 139 min compared to 76 min for methyl maltoside (1) under identical reaction conditions.

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References

  1. Lemieux RU, Wong TC, Liao J, Kabat EA (1984) Mol Immunol 21:751–59.

    Google Scholar 

  2. Spohr U, Hindsgaul O, Lemieux RU (1985) Can J Chem 63:2644–52.

    Google Scholar 

  3. Hindsgaul O, Khare DP, Bach M, Lemieux RU (1985) Can J Chem 63:2653–58.

    Google Scholar 

  4. Spohr U, Morishima N, Hindsgaul O, Lemieux RU (1985) Can J Chem 63:2659–63.

    Google Scholar 

  5. Lemieux RU, Venot AP, Spohr U, Bird P, Mandal G, Morishima N, Hindsgaul O, Bundle DR (1985) Can J Chem 63:2664–68.

    Google Scholar 

  6. Lemieux RU (1984) in Proc VII Int Symp Medicinal Chem, August 27–31 (1984) Uppsala, Sweden, p 329–51.

  7. Bock K, Pure Appl Chem, in press.

  8. Bock K, Pedersen H (1986) in Molecular Biology of Microbiological Pathogenicity, ed. Lark D, Academic Press, London, p 173–82.

    Google Scholar 

  9. Pedersen H (1985) in Enzyme Substrate Interactions. Preparation of Substrate Analogs for Amyloglucosidase. Ph.D. Thesis., Danish Technical High School, Lyngby, Denmark.

    Google Scholar 

  10. Lehman J, Schröter E (1987) Carbohydr Res 58:65–72.

    Google Scholar 

  11. Fraser-Reid B, Radatus B, Yik-Kai Tam S (1980) Methods Carbohydr Chem 8:219–25.

    Google Scholar 

  12. Thiem J, Karl H, Schwentner J (1978) Synthesis 696–98.

  13. Pearson PA (1985) J Magn Reson 64:487–500.

    Google Scholar 

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Author notes

  1. José Fernandez Bolanos Guzman

    Present address: Faculty of Chemistry, University of Sevilla, P.O. Box 553, 41071, Sevilla, Spain

Authors and Affiliations

  1. Department of Organic Chemistry, The Technical University of Denmark, DK-2800, Lyngby, Denmark

    Klaus Bock & Susanne Refn

Authors
  1. Klaus Bock
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  2. José Fernandez Bolanos Guzman
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  3. Susanne Refn
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Bock, K., Guzman, J.F.B. & Refn, S. The substrate specificity of the enzyme amyloglucosidase towards a pentadeoxy derivative of maltose. Glycoconjugate J 4, 283–290 (1987). https://doi.org/10.1007/BF01048434

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  • DOI: https://doi.org/10.1007/BF01048434

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