Abstract
A cultured soybean cell line, SB-1 was used to evaluate the initial interaction between the soybean cells andRhizobium japonicum. Co-culturing ofR. japonicum with SB-1 cells in suspension resulted in strain-specific polar attachment. This attachment can be inhibited by galactose and antibodies raised against seed soybean agglutinin (SBA). A lectin was purified from SB-1 cells which shares properties with SBA in terms of immunological reactivity, sugar binding activity, polypeptide molecular weight and peptide maps. When the SB-1 cells were co-cultured withR. japonicum for three weeks in solid agar medium, histological staining revealed bacterial penetration into certain SB-1 cells. Furthermore, there were focal regions of cells with prominent nuclei representing actively proliferating regions. These observations are analogous to that ofin vivo nodule initiation in soybean roots.
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Ho, SC., Malek-Hedayat, S. Binding and penetration ofRhizobium japonicum to cultured soybean cells. Glycoconjugate J 4, 391–398 (1987). https://doi.org/10.1007/BF01048372
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DOI: https://doi.org/10.1007/BF01048372