Abstract
U-937 monoblastic cells were differentiated into macrophage-like cells in the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA). Control cells and differentiated cells were labeled with35S-sulfate and were both found to produce exclusively chondroitin sulfate proteoglycan. No differences in glycosaminoglycan structure or macromolecular properties of the proteoglycans produced in the two different cell systems could be observed. However, the differentiated cells were found to have a lower capacity for chondroitin sulfate proteoglycan synthesis, both under ordinary experimental conditions, and when exposed to stimulators of glycosaminoglycan biosynthesis such as β-d-xylosides.
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Abbreviations
- SDS:
-
sodium dodecyl sulfate
- TPA:
-
12-O-tetradecanoylphorbol-13-acetate
- PG:
-
proteoglycan
- GAG:
-
glycoaminoglycan
- CS:
-
chondroitin sulfate
- CSPG:
-
chondroitin sulfate proteoglycan
- NASDAE:
-
naphthol AS-D acetate esterase
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Kolset, S.O., Ivhed, I., Øvervatn, A. et al. The expression of proteoglycans in phorbol ester induced U-937 cells. Glycoconjugate J 5, 107–118 (1988). https://doi.org/10.1007/BF01048336
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DOI: https://doi.org/10.1007/BF01048336