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Concentration of enteroviruses from seawater and tapwater by organic flocculation using non-fat dry milk and casein

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Abstract

The use of casein and non-fat dry milk for the recovery of enteroviruses from seawater and tapwater was investigated. Poliovirus type 1 was recovered with high efficiency (>70% overall recovery) from large volumes of seawater or tapwater, using 1% (w/v) non-fat dry milk buffered at pH 9.0 with 0.05M glycine. Good recovery of low numbers of poliovirus 1 from tapwater was also achieved. The method can also recover other enteroviruses (Poliovirus 2, 3, coxsackievirus B3 and echovirus 4), but appeared inefficient in the recovery of echovirus 1.

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Authors and Affiliations

  1. Department of Environmental Engineering Sciences and Department of Microbiology and Cell Sciences, University of Florida, 32611, Gainesville, FL, USA

    Gabriel Bitton, Bruce N. Feldberg & S. R. Farrah

Authors
  1. Gabriel Bitton
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  2. Bruce N. Feldberg
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  3. S. R. Farrah
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Bitton, G., Feldberg, B.N. & Farrah, S.R. Concentration of enteroviruses from seawater and tapwater by organic flocculation using non-fat dry milk and casein. Water Air Soil Pollut 12, 187–195 (1979). https://doi.org/10.1007/BF01047122

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  • DOI: https://doi.org/10.1007/BF01047122

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