Abstract
A recent development of kinetic light-scattering and -absorption photometry is described.
Essential points are:
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1)
In the scattering experiment, amplitude resolution of 2 · 10−5 (single flash) by application of a differential detector, stability of the same magnitude due to optical compensation, high intensity at the detector due to special optics for the scattered light and semiconductor sources.
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2)
In the absorption measurement, elimination of scattering contributions by the dual wavelength-method and by high aperture optics.
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3)
Simultaneous measurement of absorption and scattering. The application of the method is described in using signals from isolated bovine rod outer segments. A reliable procedure is described by the use of which the originally measured light-scattering effects can be split up into single signals.
The method allows comparative kinetic analysis of absorption and scattering signals. The possible causal connections between pigment and membrane structure processes can be selected.
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Hofmann, K.P., Emeis, D. Comparative kinetic light-scattering and -absorption photometry. Biophys. Struct. Mechanism 8, 23–34 (1981). https://doi.org/10.1007/BF01047103
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DOI: https://doi.org/10.1007/BF01047103