Summary
A combined enzyme-histochemical (ATPase reactivity) and immunohistochemical study has been performed on sections of rabbit masseter muscle. The majority of the fibres previously designated as type IIC and/or type I according to their ATPase activity were found to contain ‘cardiac’ α-myosin heavy chain in addition to other myosin heavy chains. All α-myosin heavy chain-containing fibres reveal ATPase activity after pre-incubation at pH 4.2–4.6 similar to that of the classical type I fibres, while in that pH range, limb type IIC fibres show intermediate ATPase activity. One group of these fibres reveal ATPase activity after pre-incubation at pH 10.1–10.3 as well, but not at pH 10.4–10.5. These fibres contain exclusively either α- or α- and I-myosin heavy chains but do not contain the IIA-myosin heavy chain. The second part of the fibres reveals ATPase activity after treatment within the whole alkaline pre-incubation range (pH 10.1–10.5) and these fibres contain α-myosin and IIA-myosin but no, I-myosin heavy chain.
It is concluded that the classical IIC fibre type is not present in the rabbit masseter muscle. Furthermore, ATPase reactivity does not allow us to distinguish fibres on their myosin heavy chain content in rabbit masseter muscle.
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References
D'Albis, A., Janmot, C., &Bechet, J. J. (1986) Comparison of myosins from the masseter muscle of adult rat, mouse and guinea-pig.Eur. J. Biochem. 156, 291–6.
D'Albis, A., Janmot, C., Mira, J. C. &Couteaux, R. (1991) Characterization of ventricular V1 myosin isoform in rabbit masticatory muscles. Developmental and natural regulation.BAM,1, 23–34.
Billeter, R., Weber, H., Lutz, H., Howald, H., Eppenberger, H. M. &Jenny, E. (1980). Myosin types in human skeletal muscle fibres.Histochemistry,65, 249–59.
Bredman, J. J., Weijs, W. A., Moorman, A. F. M. &Brugman, P. (1990a) Histochemical and functional fibre typing of the rabbit masseter muscle.J. Anat. 168, 31–47.
Bredman, J. J., Weijs, W. A. &Moorman, A. F. M. (1990b) Expression of ‘cardiac-specific’ myosin heavy chain in rabbit cranial muscles.Muscle and Motility 2; Proceedings of the XIX European Conference on Muscle and Motility pp. 329–35. Brussels: Intercept.
Bredman, J. J., Wessels, A., Weijs, W. A., Korfage, J. A. M., Soffers, C. A. S. &Moorman, A. F. M. (1991a) Demonstration of ‘cardiac-specific’ myosin heavy chain in masticatory muscles of human and rabbit.Histochem. J. 23, 160–170.
Bredman, J. J., Weijs, W. A., Korfage, J. A. M., Brugman, P. & Moorman, F. M (1991b) Myosin heavy chain expression in rabbit masseter muscle during postnatal development.J. Anat. (in press).
Butler-Browne, G. S., Eriksson, P. O., Laurent, C. &Thornell, L. E. (1988) Adult human masseter muscle fibres express myosin isozymes characteristics of development.Muscle Nerve 11, 610–20.
Gorza, L. (1990) Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies.J. Histochem. Cytochem. 38, 257–65.
De Groot, I. J. M., Lamers, W. H. &Moorman, A. F. M. (1989) Isomyosin expression patterns during rat heart morphogenesis: an immunohistochemical study.Anat. Rec. 224, 365–73.
Moorman, A. F. M., De Boer, P. A. J., Linders, M. TH. &Charles, R. (1984) The histone H5 variant inXenopus laevis.Cell Differ. 14, 113–23.
Rowlerson, A., Mascarello, F., Veggetti, A., &Caprenè E. (1983) The fibre-type composition of the first branchial arch muscles in carnivora and primates.J. Muscle Res. Cell Motil. 4, 443–72.
Rowlerson, A., Mascarello, F., Barker, D &Saed, H. (1988) Muscle-spindle distribution in relation to the fibre-type composition of masseter in mammals.J. Anat. 161, 37–60.
Schiaffino, S. (1974) Histochemical enzyme profile of the masseter muscle in different mammalian species.Anat. Rec. 180, 53–62.
Schiaffino, S., Gorza, L. &Sartore, S. (1989) Three myosin heavy chain isoforms in type 2 skeletal muscle fibers.J. Muscle Res. Cell Motil. 10, 197–205.
Staron, R. S. &Pette, D. (1986) Correlation between myofibrillar ATPase activity and myosin heavy chain composition in rabbit muscle fibers.Histochemistry 86, 19–23.
Staron, R. S., Hikida, R. S. &Hagerman, F. C. (1983) Reevaluation of human muscle fast-twitch subtypes: evidence for a continuum.Histochemistry 78, 33–9.
Wessels, A., Vermeulen, J. L. M., Moorman, A. F. M., &Becker, A. E. (1988) Immunohistochemical detection of myosin heavy chain isoforms in large sections of whole human hearts.Proceedings of XVIII European Conference on Muscle and Motility pp. 311–6. Padova: Unipress.
Wessels, A., Vermeulen, J. L. M., Virágh, SZ., Kálmán, F., Lamers, W. H. &Moorman, A. F. M. (1990) Spatial distribution of ‘tissue specific’ antigens in the developing human heart and skeletal muscle: (II) an immunohistochemical analysis of myosin heavy chain isoform expression patterns in the embryonic heart.Anat. Rec. 229, 355–68.
Weys, W. A., Brugman, P. &Klok, E. M. (1987) The growth of the skull and jaw muscles of the rabbit and its functional consequences in the New Zealand rabbit (Oryctolagus caniculus).J. Morphol. 194, 143–61.
Yamouchi-Takihara, K., Sole, M. J., Liew, J., Ing, D. &Liew, C. (1989) Characterization of human ‘cardiac’ myosin heavy chain genes.Proc. Natl. Acad. Sci. USA 86, 3504–8.
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Bredman, J.J., Weijs, W.A. & Moorman, A.F.M. Presence of cardiac α-myosin correlates with histochemical myosin Ca2+ ATPase activity in rabbit masseter muscle. Histochem J 24, 260–265 (1992). https://doi.org/10.1007/BF01046840
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DOI: https://doi.org/10.1007/BF01046840