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Postembedding immunogold histochemistry for the localization of laminin and its E4 and P1 fragments in mouse kidney embedded in LR-White and LR-Gold

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Summary

A method is presented which permits the ultrastructural localization of laminin and its E4 and P1 subunits in the renal cortex of the mouse embedded in LR-White or LR-Gold. It was performed with postembedding immunogold histochemistry using polyclonal antibodies against either the entire laminin molecular or the E4 fragment or with a monoclonal antibody against the P1 fragment. Localization of laminin was achieved in LR-White and in LR-Gold embedded kidney. Using polyclonal antibodies against the entire laminin molecule, laminin could be localized with direct as well as with indirect immunogold histochemistry with a gold labelled IgG as secondary antibody. In contrast, immunostaining for the E4 or the P1 fragments was possible only with antibodibodies directly labelled with gold.

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  1. Abteilung Histologie, Zentrum Anatomie, Kreuzbergring 36, D-3400, Göttingen, Germany

    R. Herken & N. Miosge

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  1. R. Herken
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  2. N. Miosge
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Herken, R., Miosge, N. Postembedding immunogold histochemistry for the localization of laminin and its E4 and P1 fragments in mouse kidney embedded in LR-White and LR-Gold. Histochem J 23, 267–272 (1991). https://doi.org/10.1007/BF01045045

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  • DOI: https://doi.org/10.1007/BF01045045

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