Summary
Genetic determinants of the Muc+ character were investigated in two ropy strains,Lactobacillus delbrueckii ssp.bulgaricus 201 andL. casei ssp.casei NCIB 4114, which secrete a large amount of slime in culture media. Plasmid DNA analysis revealed the presence of two plasmids (4.5 and 2.3 Mdal) inL. casei ssp.casei, whileL. delbrueckii ssp.bulgaricus was plasmid free, suggesting a chromosomal location of Muc+ character in this strain. Curing experiments carried out onL. casei ssp.casei NCIB 4114 indicated a correlation between the Muc+ phenotype and the 4.5 Mdal plasmid.
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Vescovo, M., Scolari, G.L. & Bottazzi, V. Plasmid-encoded ropiness production inLactobacillus casei SSP.casei . Biotechnol Lett 11, 709–712 (1989). https://doi.org/10.1007/BF01044102
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DOI: https://doi.org/10.1007/BF01044102