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Untersuchung von Cerealien auf Deoxynivalenol und Nivalenol im unteren μg/kg-Bereich

Determination of deoxynivalenol and nivalenol in cereals at the μg/kg concentrationrange

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Summary

A method is described for the determination of Deoxynivalenol (DON) and Nivalenol (NV). A liquid chromatographic clean-up step is followed by silylation of the extract and the trimethylsilylether are determined by gaschromatography-masspectrometry (GC-MS), monitoring the characteristic ions in the negative chemical ionisation mode. 71 % of 42 food-samples were contaminated with DON and 38% with NV. The highest contamination detected was 2000 μg/kg DON and 120 μg/kg NV in a wheat bran sample. Deoxynivalenol and Nivalenol in concentrations of 10 μg/kg can be detected easily by this method.

Zusammenfassung

Es wird ein Analysenverfahren zur Bestimmung von Deoxynivalenol (DON) und Nivalenol (NV) beschrieben. Die säulenchromatographisch gereinigten Extrakte werden silyliert und die Silylether durch Detektion substanzspezifischer Ionen im negativen CI-Betrieb gaschromatographisch-massenspektrometrisch bestimmt. Von 42 untersuchten Lebensmitteln waren 71% mit DON und 38% mit NV kontaminiert. Der höchste Gehalt wurde in einer Weizenkleie mit 2 000 μg/kg DON und 120 μg/kg NV festgestellt. Die Nachweisempfindlichkeit der Methode liegt bei etwa 10 μg/kg Deoxynivalenol und Nivalenol.

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Author notes

  1. Manfred Kellert

    Present address: Institut für Lebensmittelchemie der Johann-Wolfgang-Goethe-Universität, Robert-Mayer-Straße 7-9, 6000, Frankfurt am Main 1

Authors and Affiliations

  1. Max von Pettenkofer-Institut des Bundesgesundheitsamtes, D-1000, Berlin 33

    Werner Blaas, Manfred Kellert, Sigrid Steinmeyer, Reinhard Tiebach & Rudolf Weber

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  1. Werner Blaas
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  2. Manfred Kellert
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  3. Sigrid Steinmeyer
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  4. Reinhard Tiebach
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  5. Rudolf Weber
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Blaas, W., Kellert, M., Steinmeyer, S. et al. Untersuchung von Cerealien auf Deoxynivalenol und Nivalenol im unteren μg/kg-Bereich. Z Lebensm Unters Forch 179, 104–108 (1984). https://doi.org/10.1007/BF01043259

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  • DOI: https://doi.org/10.1007/BF01043259

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