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Digoxigenin as a probe label forin situ hybridization on skeletal tissues

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Summary

Non-specific staining was encountered using digoxigenin-labelled cDNA probes forin situ hybridization on sections of skeletal tissues. This staining was most pronounced in cartilaginous matrices. Experimental procedures indicate that the background staining is caused by antibody-binding to hydrophobic sites in the tissues revealed by proteolytic permeabilization. A protocol for minimizing this background is described.

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References

  • Applications Manual (1989)DNA Labeling and Detection Nonradioactive. Mannheim: Boehringer.

  • Technical Bulletin (1990) In situhybridization with tissue sections using digoxygenin-labelled oligonucleotide probes (Genius TM System). Mannehei Boehringer.

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Authors and Affiliations

  1. Department of Anatomy, St George's Hospital Medical School, Tooting, SW17 ORE, London, UK

    Yvette S. Bland, M. A. Critchlow & Doreen E. Ashhurst

Authors
  1. Yvette S. Bland
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  2. M. A. Critchlow
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  3. Doreen E. Ashhurst
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Bland, Y.S., Critchlow, M.A. & Ashhurst, D.E. Digoxigenin as a probe label forin situ hybridization on skeletal tissues. Histochem J 23, 415–418 (1991). https://doi.org/10.1007/BF01042298

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  • DOI: https://doi.org/10.1007/BF01042298

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