Summary
The 4-methoxynaphthylamide (MNA) derivative ofd-Val-Leu-Arg-4-MNA has been used as a substrate for the histochemical localization of esteroproteases in the submandibular gland of rats, and compared with the substrate αN-O-met. The hydrolysis of Val-Leu-Arg-4-MNA by esteroproteases was investigated using spectrophotometry and isoelectric focusing. Both methods demonstrated that the substrate is cleaved by different enzymes and is not a monospecific kallikrein marker, although Val-Leu-Arg-4-MNA had a much smaller spectrum of enzyme activities than αN-O-met. The activity against Val-Leu-Arg-4-MNA was totally inhibited by aprotinin but only partly by soybean trypsin inhibitor. A comparison of the histochemical localization of esteroproteases given by the substrate with the immunofluorescence localization of kallikrein showed that all the enzymes that hydrolysed Val-Leu-Arg-4-MNA and kallikrein were present in the same tissue structures.
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Arnold, W.H., Ørstavik, T.B. & Holck, M. A 4-methoxy-2-naphthylamide substrate for the histochemical localization of esteroproteases in the submandibular gland of the rat. Histochem J 15, 139–146 (1983). https://doi.org/10.1007/BF01042282
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DOI: https://doi.org/10.1007/BF01042282