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Saccharidtrennung mittels Gradientenelution

The separation of saccharides by means of a gradient elution

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Summary

The analysis of the composition of saccharide melts led to a modification of the customary HPLC method by means of NH2 columns [1–4]. For the qualitative and quantitative determination of the saccharides (within the range from monosaccharides to oligosaccharides) a gradient system was developed that permits the separation and subsequent detection of saccharides (DP1 = 1 to DP = 6) by using a differential refractometer.

Zusammenfassung

Die Untersuchung von Saccharidschmelzen auf deren Zusammensetzung führte zu einer Modifizierung der bisher üblichen HPLC-Methode mittels NH2-Säulen [1–4]. Zur qualitativen und quantitativen Erfassung von Sacchariden im Bereich von Monosacchariden bis zu Oligosacchariden wurde ein Gradientensystem entwickelt, das die Trennung der Saccharide (DP1 = 1 bis DP = 6) und deren anschließenden Nachweis mittels Differential-Refraktometer ermöglicht.

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Literatur

  1. Brous C, Olieman C (1983) J Chromatogr 259:79–86

    Google Scholar 

  2. Nikolov ZL, Jakovljevic JB, Boskov ZM (1984) Stärke 36:97–100

    Google Scholar 

  3. Ugrinovits M (1980) Chromatographia 13:386–394

    Google Scholar 

  4. Woidich H, Pfannhauser W, Blaicher G (1978) Lebensm Chem Gerichd Chem 32:74–76

    Google Scholar 

  5. Schwedt G (1984) Z Lebensm Unters Forsch 179:183–189

    Google Scholar 

  6. Conrad EC, Palmer JK (1976) Food Technol 10:84–92

    Google Scholar 

  7. Heyraud A, Rinando M (1980) J Liquid Chromatogr 3:721–739

    Google Scholar 

  8. Ivie KF (1982) Sugar y Azucar 77:45–53

    Google Scholar 

  9. Linden JK, Lawhead CL (1975) J Chromatogr 105:125–133

    Google Scholar 

  10. McGinnis GD, Prince S, Lowrimore J (1986) J Carbohydrate Chem 5:83–97

    Google Scholar 

  11. .Schwarzenbach R (1977) Mitt Geb Lebensm Hyg 68:64–71

    Google Scholar 

  12. Scobell HD, Brobst KM, Steele AE (1977) Cereal Chem 54:905–917

    Google Scholar 

  13. Vratny P, Coupek J, Vozka S, Hostomska Z (1983) J Chromatogr 254:143–155

    Google Scholar 

  14. Abeydeera WPP (1983) Int Sugar J 85:300–306

    Google Scholar 

  15. Charles DF (1981) Int Sugar J 83:169–172, 195–199

    Google Scholar 

  16. Clarke MA, Brannan MA (1980) Proc Techn Session Cane Sugar Refining Res 138–149

  17. Hunt DC, Jackson PA, Mortlock RE, Kirk RS (1977) Analyst 102:917–920

    Google Scholar 

  18. Kawamoto T, Okada E (1983) J Chromatogr 258:284–288

    Google Scholar 

  19. Palmer JK, Brandes WB (1974) J Agric Food Chem 22:709–712

    Google Scholar 

  20. Jandera P, Churacek J (1975) J Chromatogr 98:55–104

    Google Scholar 

  21. John M, Trenel G, Dellweg H (1969) J Chromatogr 42:476–484

    Google Scholar 

  22. Richter K, Woelk HU (1977) Starke 29:273–277

    Google Scholar 

  23. Galensa R (1984) Z Lebensm Unters Forsch 178:199–202

    Google Scholar 

  24. Müller H, Siepe V (1980) Dtsch Lebensm Rundsch 76:156–161

    Google Scholar 

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Authors and Affiliations

  1. Technische Universität Berlin, Institut für Lebensmitteltechnologie — Fachgebiet Zuckertechnologie, Amrumer Strasse 32, D-1000, Berlin 65

    Werner Mauch & Achim Gennrich

  2. Institut für angewandte Chromatographic, Blücherstrasse 22, D-1000, Berlin 61

    Imre Molnar

Authors
  1. Werner Mauch
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  2. Achim Gennrich
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  3. Imre Molnar
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Mauch, W., Gennrich, A. & Molnar, I. Saccharidtrennung mittels Gradientenelution. Z Lebensm Unters Forch 185, 383–385 (1987). https://doi.org/10.1007/BF01042259

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  • DOI: https://doi.org/10.1007/BF01042259

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