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Detection of p24 in HIV-1 infected cells embedded in LR White and Lowicryl K4M

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Summary

In this study we present a postembedding on-grid immunogold labelling procedure for the ultrastructural localization of the HIV-1 core protein p24. HIV-1 infected cells were fixed in 0.1% glutaraldehyde, incompletely dehydrated and embedded in LR White or in Lowicryl K4M. Antigenic sites were detected by incubation of ultrathin sections with primary mouse monoclonal antibody anti-HIV-1 p24, followed by the secondary antibody goat anti-mouse IgG coupled to 10nm gold particles. Antigenicity of p24 was found to withstand the applied fixation and was shown to be preserved in LR White as well as in Lowicryl. The described procedure permits the uncomplicated and easy detection of p24 in HIV-1 infected cells and tissues.

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Authors and Affiliations

  1. Division of Clinical Immunology and Rheumatology, Department of Medicine, The University of Alabama at Birmingham, 35294, Birmingham, Alabama, USA

    Gregor Stransky & Steffen Gay

  2. Department of Microbiology and Immunology, Tulane University School of Medicine, 70112, New Orleans, Louisians, USA

    Robert F. Garry

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  1. Gregor Stransky
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  2. Robert F. Garry
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  3. Steffen Gay
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Stransky, G., Garry, R.F. & Gay, S. Detection of p24 in HIV-1 infected cells embedded in LR White and Lowicryl K4M. Histochem J 23, 381–384 (1991). https://doi.org/10.1007/BF01042184

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  • DOI: https://doi.org/10.1007/BF01042184

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